Key features and details
- Rabbit polyclonal to Aconitase 1/ACO1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Aconitase 1/ACO1 antibody
See all Aconitase 1/ACO1 primary antibodies
DescriptionRabbit polyclonal to Aconitase 1/ACO1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Recombinant fragment corresponding to Human Aconitase 1/ACO1 aa 508-745.
GNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLV IAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPG MFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQ PPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFN SYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLP
Database link: P21399
- WB: HeLa and HepG2 whole cell lysate. IHC-P: Human kidney and small intestine tissue. ICC/IF: HeLa cells.
This product was previously labelled as Aconitase 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab236773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 98 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionIron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
- Information by UniProt
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
All lanes : Anti-Aconitase 1/ACO1 antibody (ab236773) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98 kDa
Paraffin-embedded human kidney tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 1/ACO1 (Green) using ab236773 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236773 has not yet been referenced specifically in any publications.