Overview

  • Product name

    Anti-Aconitase 1/ACO1 antibody
    See all Aconitase 1/ACO1 primary antibodies
  • Description

    Rabbit polyclonal to Aconitase 1/ACO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Aconitase 1/ACO1 aa 508-745.
    Sequence:

    GNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLV IAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPG MFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQ PPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFN SYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLP


    Database link: P21399

  • Positive control

    • WB: HeLa and HepG2 whole cell lysate. IHC-P: Human kidney and small intestine tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as Aconitase 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab236773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Predicted molecular weight: 98 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • Sequence similarities

    Belongs to the aconitase/IPM isomerase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACO 1 antibody
    • ACO1 antibody
    • ACOC_HUMAN antibody
    • Aconitase 1 soluble antibody
    • Aconitase antibody
    • Aconitase1 antibody
    • Aconitate hydratase antibody
    • ACONS antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • Cytoplasmic aconitate hydratase antibody
    • Ferritin repressor protein antibody
    • IRE BP 1 antibody
    • IRE-BP 1 antibody
    • IREB 1 antibody
    • IREB1 antibody
    • IREBP antibody
    • IREBP1 antibody
    • Iron regulatory protein 1 antibody
    • Iron responsive element binding protein 1 antibody
    • Iron-responsive element-binding protein 1 antibody
    • IRP 1 antibody
    • IRP1 antibody
    • OTTHUMP00000045233 antibody
    see all

Images

  • All lanes : Anti-Aconitase 1/ACO1 antibody (ab236773) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 98 kDa

  • Paraffin-embedded human kidney tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human small intestine tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 1/ACO1 (Green) using ab236773 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236773 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236773.
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