Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)

Overview

  • Product name

    Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]
    See all Aconitase 1/ACO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aconitase 1/ACO1 aa 750-850. The exact sequence is proprietary.
    Database link: P21399

  • Positive control

    • WB: Human fetal liver tissue lysate, HepG2, T.T, U20S, HeLa and HEK-293 whole cell lysate
  • General notes

     

     

     This product was previously labelled as Aconitase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 98 kDa.

Target

  • Function

    Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • Sequence similarities

    Belongs to the aconitase/IPM isomerase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACO 1 antibody
    • ACO1 antibody
    • ACOC_HUMAN antibody
    • Aconitase 1 soluble antibody
    • Aconitase antibody
    • Aconitase1 antibody
    • Aconitate hydratase antibody
    • ACONS antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • Cytoplasmic aconitate hydratase antibody
    • Ferritin repressor protein antibody
    • IRE BP 1 antibody
    • IRE-BP 1 antibody
    • IREB 1 antibody
    • IREB1 antibody
    • IREBP antibody
    • IREBP1 antibody
    • Iron regulatory protein 1 antibody
    • Iron responsive element binding protein 1 antibody
    • Iron-responsive element-binding protein 1 antibody
    • IRP 1 antibody
    • IRP1 antibody
    • OTTHUMP00000045233 antibody
    see all

Images

  • All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/ml

    Lane 1 : Wild-type U20S whole cell lysate
    Lane 2 : ACO1 knockout U20S whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 98 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. Ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 98 kDa

  • All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : T.T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 98 kDa

References

ab183721 has not yet been referenced specifically in any publications.

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