Recombinant Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)](https://www.abcam.com/ps/products/183/ab183721/Images/ab183721-352455-anti-aconitase-1-antibody-epr72262-western-blot-knock-out-human-lysates.jpg "Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]
See all Aconitase 1/ACO1 primary antibodies -
Description
Rabbit monoclonal [EPR7226(2)] to Aconitase 1/ACO1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver tissue lysate, HepG2, T.T, U20S, HeLa and HEK-293 whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7226(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/20000. Predicted molecular weight: 98 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/20000. Predicted molecular weight: 98 kDa. |
Target
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Function
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 48 Human
- Entrez Gene: 11428 Mouse
- Entrez Gene: 50655 Rat
- Omim: 100880 Human
- SwissProt: P21399 Human
- SwissProt: P28271 Mouse
- SwissProt: Q63270 Rat
- Unigene: 567229 Human
see all -
Alternative names
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
see all
Images
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Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/ml
Lane 1 : Wild-type U20S whole cell lysate
Lane 2 : ACO1 knockout U20S whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HEK-293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 98 kDaLanes 1 - 4: Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. Ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)](https://www.abcam.com/ps/products/183/ab183721/Images/ab183721-215741-anti-aconitase-1-antibody-epr72262-western-blot.jpg)
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 98 kDa -
![Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)](https://www.abcam.com/ps/products/183/ab183721/Images/ab183721-215739-anti-aconitase-1-antibody-epr72262-western-blot.jpg)
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)All lanes : Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : T.T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 98 kDa -
![Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)](https://www.abcam.com/ps/products/183/ab183721/Images/ab183721-4-benefits-of-recombinant-antibodies.png)
Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183721 has not yet been referenced specifically in any publications.