Anti-Aconitase 2 antibody (ab129957)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies -
Description
Rabbit polyclonal to Aconitase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Aconitase 2.
Read Abcam's proprietary immunogen policy -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Liver; Human Kidney; Human Heart; Human Heart Mitochondrial; Mouse Brain; Rat Brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
Target
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Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 374009 Chicken
- Entrez Gene: 280976 Cow
- Entrez Gene: 474487 Dog
- Entrez Gene: 101126782 Gorilla
- Entrez Gene: 100055803 Horse
- Entrez Gene: 11429 Mouse
- Entrez Gene: 396999 Pig
- Entrez Gene: 100352714 Rabbit
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
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Western blot - Anti-Aconitase 2 antibody (ab129957)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab129957 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129957 was shown to recognize Aconitase 2 in wild-type HAP1 cells as signal was lost at the expected MW in Aconitase 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Aconitase 2 antibody (ab129957)All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human Heart Mitochondrial Lysate
Lane 6 : Brain (Mouse) Tissue Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Metodiev MD et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet 51:834-8 (2014). Read more (PubMed: 25351951) »
