Product nameAnti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies
DescriptionRabbit polyclonal to Aconitase 2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Aconitase 2.
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Liver; Human Kidney; Human Heart; Human Heart Mitochondrial; Mouse Brain; Rat Brain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
FunctionCatalyzes the isomerization of citrate to isocitrate via cis-aconitate.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
- Information by UniProt
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129957 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129957 was shown to recognize Aconitase 2 in wild-type HAP1 cells as signal was lost at the expected MW in Aconitase 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human Heart Mitochondrial Lysate
Lane 6 : Brain (Mouse) Tissue Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This product has been referenced in:
- Metodiev MD et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet 51:834-8 (2014). Read more (PubMed: 25351951) »