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Rabbit polyclonal to Aconitase 2
Synthetic peptide (Human) from the N-terminus conjugated to KLH
Rat heart whole tissue lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Belongs to the aconitase/IPM isomerase family.
Information by UniProt
ACO 2 antibody
Western blot - Anti-Aconitase 2 antibody (ab71439)
All lanes : Anti-Aconitase 2 antibody (ab71439) at 1/1000 dilution Lane 1 : Rat heart whole tissue lysate lysed with 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail Lane 2 : Rat heart whole tissue lysate lysed with T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail Lysates/proteins at 12.5 µg per lane. Predicted band size: 85 kDa Observed band size: 85 kDa
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