Anti-Aconitase 2 antibody (ab99467)
Key features and details
- Goat polyclonal to Aconitase 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies -
Description
Goat polyclonal to Aconitase 2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
C-QHVDVSPTSQRLQ
, corresponding to internal sequence amino acids 554-566 of Human Aconitase 2 (NP_001089.1). -
Positive control
- Human Heart lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99467 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 280976 Cow
- Entrez Gene: 474487 Dog
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 396999 Pig
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: P20004 Cow
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab99467 has been referenced in 2 publications.
- Márquez-Jurado S et al. Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression. Nat Commun 9:389 (2018). PubMed: 29374163
- Morton NM et al. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med 22:771-9 (2016). PubMed: 27270587