Key features and details
- Goat polyclonal to Aconitase 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Aconitase 2 antibody
See all Aconitase 2 primary antibodies
DescriptionGoat polyclonal to Aconitase 2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- Human Heart lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99467 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab99467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa.
1 hour primary incubation is recommended for this product.
FunctionCatalyzes the isomerization of citrate to isocitrate via cis-aconitate.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
- Information by UniProt
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
Anti-Aconitase 2 antibody (ab99467) at 0.01 µg/ml + Human Heart lysates at 35 µg
Predicted band size: 85 kDa
ab99467 (3.8µg/ml) staining of paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab99467 has been referenced in 2 publications.
- Márquez-Jurado S et al. Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression. Nat Commun 9:389 (2018). PubMed: 29374163
- Morton NM et al. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med 22:771-9 (2016). PubMed: 27270587