Recombinant Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-299876-anti-aconitase-2-antibody-epr8282b-western-blot.jpg "Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8282(B)] to Aconitase 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Aconitase 2 antibody [EPR8282(B)]
See all Aconitase 2 primary antibodies -
Description
Rabbit monoclonal [EPR8282(B)] to Aconitase 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human Aconitase 2 aa 50-150. The exact sequence is proprietary.
-
Positive control
- HeLa, K562 and Jurkat cell lysates; Human fetal heart lysate; Human kidney tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8282(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
-
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143011-anti-aconitase-2-antibody-epr8282b-western-blot.jpg)
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143010-anti-aconitase-2-antibody-epr8282b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143009-anti-aconitase-2-antibody-epr8282b-western-blot.png)
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAll lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution
Lane 1 : 1 Month old Mouse Liver
Lanes 2-3 : 3 Month old Mouse Liver
Lane 4 : 6 Month old Mouse Liver
Secondary
All lanes : Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 1 minuteBlocking: 5% Milk at 24°C for 1 Hour
-
![OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143007-anti-aconitase-2-antibody-epr8282b.jpg)
OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) -
![Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-7-benefits-of-recombinant-antibodies.png)
Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
Protocols
Datasheets and documents
References (10)
ab129069 has been referenced in 10 publications.
- Du J et al. Identification of Frataxin as a regulator of ferroptosis. Redox Biol 32:101483 (2020). PubMed: 32169822
- Fukada M et al. Identification of novel compound heterozygous mutations in ACO2 in a patient with progressive cerebral and cerebellar atrophy. Mol Genet Genomic Med 7:e00698 (2019). PubMed: 31106992
- Majmudar JD et al. 4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function. Medchemcomm 10:209-220 (2019). PubMed: 30881609
- Pulos-Holmes MC et al. Repression of ferritin light chain translation by human eIF3. Elife 8:N/A (2019). PubMed: 31414986
- Dukinfield M et al. Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease. J Pathol 249:523-535 (2019). PubMed: 31424556
- Jiménez-Altayó F et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). PubMed: 29471108
- Jin X et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). PubMed: 28487965
- Lu YW et al. Defining functional classes of Barth syndrome mutation in humans. Hum Mol Genet 25:1754-70 (2016). PubMed: 26908608
- Morton NM et al. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med 22:771-9 (2016). PubMed: 27270587
- McDonagh B et al. Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. J Proteome Res 13:5008-21 (2014). WB ; Mouse . PubMed: 25181601
