Product nameAnti-Aconitase 2 antibody [EPR8282(B)]
See all Aconitase 2 primary antibodies
DescriptionRabbit monoclonal [EPR8282(B)] to Aconitase 2
Tested applicationsSuitable for: IHC-Fr, WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Aconitase 2 aa 50-150. The exact sequence is proprietary.
- HeLa, K562 and Jurkat cell lysates; Human fetal heart lysate; Human kidney tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.90 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionCatalyzes the isomerization of citrate to isocitrate via cis-aconitate.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
- Information by UniProt
- Aco 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution
Lane 1 : 1 Month old Mouse Liver
Lanes 2-3 : 3 Month old Mouse Liver
Lane 4 : 6 Month old Mouse Liver
All lanes : Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 1 minute
Blocking: 5% Milk at 24°C for 1 Hour
IHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles us ab129069 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129069) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Jiménez-Altayó F et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). Read more (PubMed: 29471108) »
- Jin X et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). Read more (PubMed: 28487965) »