Overview

  • Product name
    Anti-Aconitase 2 antibody [EPR8282(B)]
    See all Aconitase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8282(B)] to Aconitase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aconitase 2 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • HeLa, K562 and Jurkat cell lysates; Human fetal heart lysate; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at  1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 85 kDa

    • ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution

      Lane 1 : 1 Month old Mouse Liver
      Lanes 2-3 : 3 Month old Mouse Liver
      Lane 4 : 6 Month old Mouse Liver

      Secondary
      All lanes : Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 85 kDa


      Exposure time: 1 minute


      Blocking: 5% Milk at 24°C for 1 Hour

      See Abreview

    • IHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles us ab129069 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129069) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Jiménez-Altayó F  et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). Read more (PubMed: 29471108) »
    • Jin X  et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). Read more (PubMed: 28487965) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocyte)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    10 µg
    Specification
    Cardiomyocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Dr. Louise Reynolds

    Verified customer

    Submitted Jun 19 2019

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse liver)
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris Gel)
    Loading amount
    10 µg
    Specification
    mouse liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 02 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela, Jurkat)
    Gel Running Conditions
    Reduced Denaturing (4-15% BisTris Gel)
    Loading amount
    15 µg
    Specification
    Hela, Jurkat
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 02 2019

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 24 2016

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 24 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle (GTN))
    Specification
    Skeletal muscle (GTN)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Skeletal muscles)
    Specification
    Skeletal muscles
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Liver Mitochondrial fraction)
    Loading amount
    20 µg
    Specification
    Liver Mitochondrial fraction
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 14 2012

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