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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

  • Datasheet
Reviews (8) Submit a question References (10)

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Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
  • Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8282(B)] to Aconitase 2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Aconitase 2 antibody [EPR8282(B)]
    See all Aconitase 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8282(B)] to Aconitase 2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Aconitase 2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562 and Jurkat cell lysates; Human fetal heart lysate; Human kidney tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.90 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8282(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Aconitase 2 protein (ab114378)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (7)
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
    • Sequence similarities

      Belongs to the aconitase/IPM isomerase family.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession Q99798 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 50 Human
      • Entrez Gene: 11429 Mouse
      • Entrez Gene: 79250 Rat
      • Omim: 100850 Human
      • SwissProt: Q99798 Human
      • SwissProt: Q99KI0 Mouse
      • SwissProt: Q9ER34 Rat
      • Unigene: 643610 Human
      • Unigene: 154581 Mouse
      • Unigene: 43737 Rat
      see all
    • Alternative names

      • ACO 2 antibody
      • ACO2 antibody
      • ACON_HUMAN antibody
      • aconitase 2 antibody
      • Aconitase 2 mitochondrial antibody
      • Aconitase antibody
      • Aconitase2 antibody
      • Aconitate hydratase antibody
      • Aconitate hydratase mitochondrial antibody
      • ACONM antibody
      • Citrate hydro lyase antibody
      • Citrate hydro-lyase antibody
      • ICRD antibody
      • mitochondrial antibody
      see all

    Images

    • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at  1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 85 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

      ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
      All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution

      Lane 1 : 1 Month old Mouse Liver
      Lanes 2-3 : 3 Month old Mouse Liver
      Lane 4 : 6 Month old Mouse Liver

      Secondary
      All lanes : Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 85 kDa


      Exposure time: 1 minute


      Blocking: 5% Milk at 24°C for 1 Hour

      See Abreview

    • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
      Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (10)

    Publishing research using ab129069? Please let us know so that we can cite the reference in this datasheet.

    ab129069 has been referenced in 10 publications.

    • Du J  et al. Identification of Frataxin as a regulator of ferroptosis. Redox Biol 32:101483 (2020). PubMed: 32169822
    • Fukada M  et al. Identification of novel compound heterozygous mutations in ACO2 in a patient with progressive cerebral and cerebellar atrophy. Mol Genet Genomic Med 7:e00698 (2019). PubMed: 31106992
    • Majmudar JD  et al. 4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function. Medchemcomm 10:209-220 (2019). PubMed: 30881609
    • Pulos-Holmes MC  et al. Repression of ferritin light chain translation by human eIF3. Elife 8:N/A (2019). PubMed: 31414986
    • Dukinfield M  et al. Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease. J Pathol 249:523-535 (2019). PubMed: 31424556
    • Jiménez-Altayó F  et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). PubMed: 29471108
    • Jin X  et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). PubMed: 28487965
    • Lu YW  et al. Defining functional classes of Barth syndrome mutation in humans. Hum Mol Genet 25:1754-70 (2016). PubMed: 26908608
    • Morton NM  et al. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med 22:771-9 (2016). PubMed: 27270587
    • McDonagh B  et al. Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. J Proteome Res 13:5008-21 (2014). WB ; Mouse . PubMed: 25181601

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocyte)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    10 µg
    Specification
    Cardiomyocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Louise Reynolds

    Verified customer

    Submitted Jun 19 2019

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse liver)
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris Gel)
    Loading amount
    10 µg
    Specification
    mouse liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2019

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela, Jurkat)
    Gel Running Conditions
    Reduced Denaturing (4-15% BisTris Gel)
    Loading amount
    15 µg
    Specification
    Hela, Jurkat
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2019

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2016

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2016

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle (GTN))
    Specification
    Skeletal muscle (GTN)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Skeletal muscles)
    Specification
    Skeletal muscles
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8282(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Liver Mitochondrial fraction)
    Loading amount
    20 µg
    Specification
    Liver Mitochondrial fraction
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 14 2012

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