Recombinant Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-Aconitase 2 antibody [EPR8282(B)]
See all Aconitase 2 primary antibodies -
Description
Rabbit monoclonal [EPR8282(B)] to Aconitase 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Aconitase 2 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, K562 and Jurkat cell lysates; Human fetal heart lysate; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8282(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/500. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/50 - 1/100. |
Target
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Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
Sequence similarities
Belongs to the aconitase/IPM isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
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![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-299876-anti-aconitase-2-antibody-epr8282b-western-blot.jpg)
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143011-anti-aconitase-2-antibody-epr8282b-western-blot.jpg)
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143010-anti-aconitase-2-antibody-epr8282b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143009-anti-aconitase-2-antibody-epr8282b-western-blot.png)
Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAll lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution
Lane 1 : 1 Month old Mouse Liver
Lanes 2-3 : 3 Month old Mouse Liver
Lane 4 : 6 Month old Mouse Liver
Secondary
All lanes : Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 1 minuteBlocking: 5% Milk at 24°C for 1 Hour
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![Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143008-anti-aconitase-2-antibody-epr8282b-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomIHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles us ab129069 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129069) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM -
![Other - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)](https://www.abcam.com/ps/products/129/ab129069/Images/ab129069-143007-anti-aconitase-2-antibody-epr8282b.jpg)
Other - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Fukada M et al. Identification of novel compound heterozygous mutations in ACO2 in a patient with progressive cerebral and cerebellar atrophy. Mol Genet Genomic Med 7:e00698 (2019). Read more (PubMed: 31106992) »
- Jiménez-Altayó F et al. Redox stress in Marfan syndrome: Dissecting the role of the NADPH oxidase NOX4 in aortic aneurysm. Free Radic Biol Med 118:44-58 (2018). Read more (PubMed: 29471108) »
