Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Reviews (3) Submit a question References (4)
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
  • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
  • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
  • Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8283(B)] to Aconitase 2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Aconitase 2 antibody [EPR8283(B)]
    See all Aconitase 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8283(B)] to Aconitase 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB (1)
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

      All lanes : Wild-type HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 85 kDa
      Observed band size: 85 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab129105 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab129105 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Hela knockout cells. Wild-type and Hela knockout samples were subjected to SDS-PAGE. Ab129105 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 85 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

      ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
      All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

      Lane 1 : 1 month old Mouse Liver Mitochondrial fraction
      Lanes 2-3 : 3 month old Mouse Liver Mitochondrial fraction
      Lane 4 : 6 month old Mouse Liver Mitochondrial fraction

      Secondary
      All lanes : Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 85 kDa


      Exposure time: 1 minute


      Blocking: 5% milk for 1 hour at 24°C

      See Abreview

    • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
      Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

    References (4)

    Publishing research using ab129105? Please let us know so that we can cite the reference in this datasheet.

    ab129105 has been referenced in 4 publications.

    • Kuter K  et al. Astrocyte support is important for the compensatory potential of the nigrostriatal system neurons during early neurodegeneration. J Neurochem 148:63-79 (2019). PubMed: 30295916
    • Sacoman JL  et al. Mitochondrial O-GlcNAc Transferase (mOGT) Regulates Mitochondrial Structure, Function, and Survival in HeLa Cells. J Biol Chem 292:4499-4518 (2017). PubMed: 28100784
    • Chi W  et al. Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye. J Autoimmun 80:65-76 (2017). PubMed: 28238526
    • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB ; Human . PubMed: 27661117

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aconitase 2 antibody [EPR8283(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Liver)
    Specification
    Liver
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Dec 11 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Aconitase 2 antibody [EPR8283(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Skeletal muscle)
    Specification
    Skeletal muscle
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

    Western blot abreview for Anti-Aconitase 2 antibody [EPR8283(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Liver Mitochondrial fraction)
    Loading amount
    20 µg
    Specification
    Liver Mitochondrial fraction
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

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