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Rabbit monoclonal [EPR8283(B)] to Aconitase 2
IHC-Fr more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.
HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Belongs to the aconitase/IPM isomerase family.
Information by UniProt
ACO 2 antibody
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution Lane 1 : HeLa cell lysate Lane 2 : K562 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : 293T cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
All lanes :
Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution
Lane 1 :
1 month old Liver Mitochondrial fraction
Lanes 2-3 :
3 month old Liver Mitochondrial fraction
Lane 4 :
6 month old Liver Mitochondrial fraction
Secondary All lanes :
Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Blocking: 5% milk for 1 hour at 24°C
Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
IHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles using ab129105 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129105) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM.
Other - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"