Recombinant
Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Aconitase 2 antibody [EPR8283(B)]
See all Aconitase 2 primary antibodies -
DescriptionRabbit monoclonal [EPR8283(B)] to Aconitase 2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cyt, IHC-Frmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.
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Positive control
- HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 2.60 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR8283(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab129105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/500. |
Application notes
Is unsuitable for IP.
Target
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FunctionCatalyzes the isomerization of citrate to isocitrate via cis-aconitate.
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PathwayCarbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
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Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
Alternative names
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
Images
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![Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)](https://www.abcam.com/ps/products/129/ab129105/Images/Aconitase-2-Primary-antibodies-ab129105-1.jpg)
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)](https://www.abcam.com/ps/products/129/ab129105/Images/Aconitase-2-Primary-antibodies-ab129105-4.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry. -
![Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)](https://www.abcam.com/ps/products/129/ab129105/Images/Aconitase-2-Primary-antibodies-ab129105-119.jpg)
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAll lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution
Lane 1 : 1 month old Liver Mitochondrial fraction
Lanes 2-3 : 3 month old Liver Mitochondrial fraction
Lane 4 : 6 month old Liver Mitochondrial fraction
Secondary
All lanes : Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 1 minuteBlocking: 5% milk for 1 hour at 24°C
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![Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)](https://www.abcam.com/ps/products/129/ab129105/Images/Aconitase-2-Primary-antibodies-ab129105-124.jpg)
Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomIHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles using ab129105 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129105) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM. -
![Other - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)](https://www.abcam.com/ps/products/129/ab129105/Images/Aconitase-2-Primary-antibodies-ab129105-130.jpg)
Other - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
Protocols
References
This product has been referenced in:
- Yu J et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB ; Human . Read more (PubMed: 27661117) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"