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Inquiry: We are using the Abcam Aconitase Assay Kit (ab83459). Could you explain why the assay isn't normalized to the amount of protein being used? Should the data be reflected as aconitase activity/mg protein? Thanks
Asked on Feb 14 2014
The mU/ml shows activity of Aconitase per unit volume. Another way of expressing the numbers will be if you perform a protein quantitation assay and instead of the ml volume of sample, uses mg of total protein. In this scenario the final numbers can be reported as mU/mg protein. Both of these are equally valid.
To determine protein mass we suggest using a detergent compatible BCA assay for protein quantitation.
Jeremy KasanovAbcam Scientific Support
Answered on Feb 14 2014