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Inquiry: Hi! What is the deal with adding the 'activation mixture' (cysteine and NH4FeSO4) to activate aconitase? A majority of studies including all references in your data sheet are looking at aconitase inactivation as a marker of ROS. If we activate aconitase by this solution what is the point of the measurment?
Asked on Sep 23 2014
The reason for needing to activate the aconitase in the sample is as follows:
The Iron sulphur cluster required for Aconitase activity is inactivated in biological samples when the aconitase is exposed to oxidants/superoxides. The way to measure Aconitase activity in vitro requires reinsertion of ferrous iron into the iron sulphur cluster. This is why you need to use the activation solution, so that the Aconitase in the samples can be tested for activity.
This is a well-studied and documented process reported in literature:
Sam WasherAbcam Scientific Support
Answered on Sep 23 2014