Product nameAnti-ACOT1 antibody
See all ACOT1 primary antibodies
DescriptionRabbit polyclonal to ACOT1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Recombinant fragment, corresponding to amino acids 183-421 of Human ACOT1 (BC132889).
- Human Fetal Skin, Liver, Heart and Thymus IF/ICC: HeLa cell line
FormLyophilised:Reconstitute in 200µl sterile water
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab100915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/500.|
RelevanceAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.
- ACH2 antibody
- Acyl CoA thioesterase 1, cytosolic antibody
- Acyl coenzyme A thioesterase 1 antibody
All lanes : Anti-ACOT1 antibody (ab100915) at 1/1000 dilution
Lane 1 : Human Fetal Skin Lysate
Lane 2 : Human Fetal Liver Lysate
Lane 3 : Human Fetal Heart Lysate
Predicted band size: 46 kDa
ab100915, at 1/100 dilution, staining cytoplasmic ACOT1 in formalin-fixed, paraffin-embedded Human fetal thymus tissue, by Immunohistochemistry.
ICC/IF image of ab100915 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100915, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Simsek D et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). Read more (PubMed: 28575669) »
- Jeckel KM et al. Dietary fatty acids alter left ventricular myocardial gene expression in Wistar rats. Nutr Res 34:694-706 (2014). WB ; Rat . Read more (PubMed: 25172377) »