Overview

  • Product name

  • Description

    Rabbit polyclonal to ACOT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ACOT1 aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156056)

  • Positive control

    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Liver; Mouse Liver; Rat Liver; Human Heart; Rat Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human Heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab133948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • Relevance

    Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • ACH2 antibody
    • Acyl CoA thioesterase 1, cytosolic antibody
    • Acyl coenzyme A thioesterase 1 antibody
    • CTE 1 antibody
    • CTE I antibody
    • CTE Ib antibody
    • Cte1 antibody
    • Cytosolic acyl CoA thioesterase 1 antibody
    • D12Ucla1 antibody
    • Inducible cytosolic acyl-coenzyme A thioester hydrolase antibody
    • Long chain acyl CoA hydrolase antibody
    • Long chain acyl CoA thioester hydrolase antibody
    see all

Images

  • IHC image of ACOT1 staining in Human Heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab133948, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-ACOT1 antibody (ab133948) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Heart (Rat) Tissue Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 46 kDa
    Additional bands at: 155 kDa, 28 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133948 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Makwana K  et al. Calorie restriction reprograms diurnal rhythms in protein translation to regulate metabolism. FASEB J 33:4473-4489 (2019). Read more (PubMed: 30566374) »
  • Wang F  et al. ACOT1 expression is associated with poor prognosis in gastric adenocarcinoma. Hum Pathol 77:35-44 (2018). IHC-P ; Human . Read more (PubMed: 29555575) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Mar 13 2018

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