Anti-ACOT12 antibody (ab196783)
Key features and details
- Rabbit polyclonal to ACOT12
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ACOT12 antibody -
Description
Rabbit polyclonal to ACOT12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human ACOT12 (internal sequence).
Database link: Q8WYK0 -
Positive control
- Human liver carcinoma tissue; A549 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab196783 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/500.
|
Notes |
---|
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/500. |
Target
-
Function
Hydrolyzes acetyl-CoA to acetate and CoA. -
Pathway
Carbohydrate metabolism; pyruvate metabolism. -
Sequence similarities
Contains 2 acyl coenzyme A hydrolase domains.
Contains 1 START domain. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 134526 Human
- Entrez Gene: 74156 Mouse
- Omim: 614315 Human
- SwissProt: Q8WYK0 Human
- SwissProt: Q9DBK0 Mouse
- Unigene: 591756 Human
- Unigene: 275963 Mouse
-
Alternative names
- ACO12_HUMAN antibody
- Acot12 antibody
- Acyl CoA thioester hydrolase 12 antibody
see all
Images
-
Immunofluorescent analysis of A549 cells labeling ACOT12 with ab196783 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
-
Immunohistochemical analysis of paraffin-embedded Human liver carcinoma tissue labeling ACOT12 with ab196783 at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab196783 has not yet been referenced specifically in any publications.