Product nameAnti-ACOT12 antibody
See all ACOT12 primary antibodies
DescriptionRabbit polyclonal to ACOT12
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- 721_B cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83326 is purified by peptide affinity chromatography method.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab83326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titer in peptide based ELISA is 1:12500.
FunctionHydrolyzes acetyl-CoA to acetate and CoA.
PathwayCarbohydrate metabolism; pyruvate metabolism.
Sequence similaritiesContains 2 acyl coenzyme A hydrolase domains.
Contains 1 START domain.
- Information by UniProt
- ACO12_HUMAN antibody
- Acot12 antibody
- Acyl CoA thioester hydrolase 12 antibody
Anti-ACOT12 antibody (ab83326) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
ab83326 has not yet been referenced specifically in any publications.