Recombinant Anti-ACOT7 antibody [EPR11317(B)] (ab156576)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11317(B)] to ACOT7
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACOT7 antibody [EPR11317(B)]
See all ACOT7 primary antibodies -
Description
Rabbit monoclonal [EPR11317(B)] to ACOT7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain, A375, 293T, and Jurkat cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11317(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IP
1/100 - 1/500. |
Target
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Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA. -
Tissue specificity
Isoform 4 is expressed exclusively in brain. -
Sequence similarities
Contains 2 acyl coenzyme A hydrolase domains. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 11332 Human
- Entrez Gene: 70025 Mouse
- Entrez Gene: 26759 Rat
- Omim: 602587 Human
- SwissProt: O00154 Human
- SwissProt: Q91V12 Mouse
- SwissProt: Q64559 Rat
- Unigene: 126137 Human
see all -
Alternative names
- ACH1 antibody
- ACOT 7 antibody
- ACOT7 antibody
see all
Images
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All lanes : Anti-ACOT7 antibody [EPR11317(B)] (ab156576) at 1/1000 dilution
Lane 1 : fetal brain cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
WB analysis of immunoprecipites
ab156576, at a 1/1000 dilution, staining ACOT7 in Jurkat cell lysate immunoprecipitated using ab156576 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156576 has not yet been referenced specifically in any publications.