Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11317(B)] to ACOT7
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-ACOT7 antibody [EPR11317(B)]
See all ACOT7 primary antibodies
DescriptionRabbit monoclonal [EPR11317(B)] to ACOT7
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human ACOT7 (UniProt ID: O00154).
- Fetal brain, A375, 293T, and Jurkat cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IP||1/100 - 1/500.|
FunctionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA.
Tissue specificityIsoform 4 is expressed exclusively in brain.
Sequence similaritiesContains 2 acyl coenzyme A hydrolase domains.
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
- ACH1 antibody
- ACOT 7 antibody
- ACOT7 antibody
All lanes : Anti-ACOT7 antibody [EPR11317(B)] (ab156576) at 1/1000 dilution
Lane 1 : fetal brain cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg/ml per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
WB analysis of immunoprecipites
ab156576, at a 1/1000 dilution, staining ACOT7 in Jurkat cell lysate immunoprecipitated using ab156576 at 1/100 dilution.
ab156576 has not yet been referenced specifically in any publications.