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RecombinantRabMAb

Recombinant Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)

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  • Certificate of Compliance
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Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
  • Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
  • Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11317(B)] to ACOT7 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Protein
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Recombinant Human ACOT7 protein (ab123169)

View more associated products

Overview

  • Product name

    Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free
    See all ACOT7 primary antibodies
  • Description

    Rabbit monoclonal [EPR11317(B)] to ACOT7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACOT7. The exact sequence is proprietary.
    Database link: O00154

  • General notes

    Ab249270 is the carrier-free version of ab156576. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249270 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11317(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation

Associated products

  • Alternative Versions

    • Anti-ACOT7 antibody [EPR11317(B)] (ab156576)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ACOT7 protein (ab123169)
  • Related Products

    • Recombinant Human ACOT7 protein (ab123169)

Applications

Our Abpromise guarantee covers the use of ab249270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA.
    • Tissue specificity

      Isoform 4 is expressed exclusively in brain.
    • Sequence similarities

      Contains 2 acyl coenzyme A hydrolase domains.
    • Cellular localization

      Cytoplasm and Mitochondrion.
    • Target information above from: UniProt accession O00154 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 11332 Human
      • Entrez Gene: 70025 Mouse
      • Entrez Gene: 26759 Rat
      • Omim: 602587 Human
      • SwissProt: O00154 Human
      • SwissProt: Q91V12 Mouse
      • SwissProt: Q64559 Rat
      • Unigene: 126137 Human
      • Unigene: 296191 Mouse
      • Unigene: 6024 Rat
      see all
    • Alternative names

      • ACH1 antibody
      • ACOT 7 antibody
      • ACOT7 antibody
      • ACT antibody
      • Acyl CoA thioesterase 7 antibody
      • Acyl CoA thioesterase long chain antibody
      • Acyl-CoA thioesterase 7 antibody
      • BACH antibody
      • BACH_HUMAN antibody
      • Brain acyl CoA hydrolase antibody
      • Brain acyl-CoA hydrolase antibody
      • CTE-II antibody
      • CTE-IIa antibody
      • Cytosolic acyl coenzyme A thioester hydrolase antibody
      • hBACH antibody
      • LACH antibody
      • LACH1 antibody
      • Long chain acyl-CoA thioester hydrolase antibody
      see all

    Images

    • Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
      Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
      All lanes : Anti-ACOT7 antibody [EPR11317(B)] (ab156576) at 1/1000 dilution

      Lane 1 : fetal brain cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa



      This data was developed using ab156576, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
      Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
      This data was developed using ab156576, the same antibody clone in a different buffer formulation.WB analysis of immunoprecipites
      ab156576, at a 1/1000 dilution, staining ACOT7 in Jurkat cell lysate immunoprecipitated using ab156576 at 1/100 dilution.
    • Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)
      Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (ab249270)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab249270? Please let us know so that we can cite the reference in this datasheet.

    ab249270 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab249270.
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