Overview

  • Product name
    Anti-ACOX1/AOX antibody [EPR19038]
    See all ACOX1/AOX primary antibodies
  • Description
    Rabbit monoclonal [EPR19038] to ACOX1/AOX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse ACOX1/AOX aa 1-300. The exact sequence is proprietary.
    Database link: Q9R0H0

  • Positive control
    • WB: Mouse liver, heart and kidney lysates; C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat brain, heart and liver lysates; Human fetal liver and fetal kidney lysates. ICC/IF: C6 and NIH/3T3 cells. Flow Cyt: NIH/3T3 cells. IP: NIH/3T3 whole cell lysate.
  • General notes

    Previously labelled as ACOX1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.
WB 1/1000. Detects a band of approximately 72, 50 kDa (predicted molecular weight: 75 kDa).
ICC/IF 1/250.
IP 1/30.

Target

  • Function
    Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
  • Tissue specificity
    Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.
  • Pathway
    Lipid metabolism; peroxisomal fatty acid beta-oxidation.
  • Involvement in disease
    Adrenoleukodystrophy, pseudoneonatal
  • Sequence similarities
    Belongs to the acyl-CoA oxidase family.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACOX antibody
    • ACOX1 antibody
    • ACOX1_HUMAN antibody
    • Acyl CoA oxidase 1 palmitoyl antibody
    • Acyl CoA oxidase straight chain antibody
    • AOX antibody
    • EC 1.3.3.6 antibody
    • PALMCOX antibody
    • Palmitoyl CoA oxidase antibody
    • Palmitoyl-CoA oxidase antibody
    • Peroxisomal acyl coenzyme A oxidase 1 antibody
    • Peroxisomal acyl-coenzyme A oxidase 1 antibody
    • Peroxisomal fatty acyl CoA oxidase antibody
    • SCOX antibody
    • Straight chain acyl CoA oxidase antibody
    • Straight-chain acyl-CoA oxidase antibody
    see all

Images

  • Lanes 1-5 : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution
    Lanes 6-7 : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/5000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa
    why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time Lane 1-5: 1 second; Lane 6: 30 seconds; Lane 7: 2 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • All lanes : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time Lane 1/2: 8 seconds;Lane 3: 1 second.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • All lanes : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast) cell line labeling ACOX1/AOX with ab184032 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR19038] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • ACOX1/AOX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab184032 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184032 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).
    Lane 2: ab184032 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR19038] - Isotype Control (ab172730) instead of ab184032 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

References

This product has been referenced in:
  • Li X  et al. Paternal hyperglycemia induces transgenerational inheritance of susceptibility to hepatic steatosis in rats involving altered methylation on Ppara promoter. Biochim Biophys Acta Mol Basis Dis 1865:147-160 (2019). Read more (PubMed: 30404040) »
  • Zhang L  et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice. Mol Nutr Food Res 62:e1800315 (2018). Read more (PubMed: 29883529) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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