Anti-Acrolein antibody [10A10] (ab240918)
![Immunohistochemistry - Anti-Acrolein antibody [10A10] (ab240918)](https://www.abcam.com/ps/products/240/ab240918/Images/ab240918-449171-anti-acrolein-antibody-10a10-immunocytochemistry-human-adrenal-carcinoma.png "Immunohistochemistry - Anti-Acrolein antibody [10A10] (ab240918)")
Key features and details
- Mouse monoclonal [10A10] to Acrolein
- Suitable for: IHC, Flow Cyt, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-Acrolein antibody [10A10]
See all Acrolein primary antibodies -
Description
Mouse monoclonal [10A10] to Acrolein -
Host species
Mouse -
Specificity
Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
-
Tested applications
Suitable for: IHC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Acrolein conjugated to keyhole limpet haemocyanin.
-
Positive control
- WB: Acrolein-BSA; HeLa cell lysate. Flow Cyt: SH-SY5Y cells. IHC: Human adrenal carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10A10 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC |
1/100.
|
|
| Flow Cyt |
1/50.
|
|
| WB |
1/1000.
|
| Notes |
|---|
|
IHC
1/100. |
|
Flow Cyt
1/50. |
|
WB
1/1000. |
Target
-
Relevance
Acrolein, a reactive alpha,beta-unsaturated aldehyde, is a common environmental pollutant, a metabolite of the anticancer drug cyclophosphamide, and a byproduct of lipid peroxidation. An increase in acrolein production has been proposed as a marker for Alzheimer's disease, diabetic glomerular lesions, and atherosclerosis. Acrolein is a potent inhibitor of cell proliferation at nonlethal doses and may act through effects on redox-regulated transcription factors. It has been reported that NF-kappaB activation is inhibited by acrolein. -
Alternative names
- 2-Propen-1-one antibody
- 2-propenal antibody
- Acraldehyde antibody
see all
Images
-
Immunohistochemistry - Anti-Acrolein antibody [10A10] (ab240918)
Immunohistochemistry analysis labeling human adrenal carcinoma tissue with ab240918 at a 1/100 dilution. Tissue samples were incubated with the primary antibody ab240918 at a 1/100 dilution overnight at 4oC. A Goat anti-Mouse IgG (H&L):FITC secondary was used (45 min at 37oC). Tissue samples were counterstained with DAPI (blue) for 3 minutes at room temperature. Magification 5X.
-
![Western blot - Anti-Acrolein antibody [10A10] (ab240918)](https://www.abcam.com/ps/products/240/ab240918/Images/ab240918-319767-anti-acrolein-antibody-10a10-western-blot.jpg)
Western blot - Anti-Acrolein antibody [10A10] (ab240918)All lanes : Anti-Acrolein antibody [10A10] (ab240918) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Hydrogen peroxide-treated HeLa cell lysate
Lysates/proteins at 12 µg per lane.
Secondary
All lanes : HRP-linked goat anti-mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlock: 5% Skim Milk in TBST.
Primary incubation for 2 hours at RT.
-
![Flow Cytometry - Anti-Acrolein antibody [10A10] (ab240918)](https://www.abcam.com/ps/products/240/ab240918/Images/ab240918-319766-anti-acrolein-antibody-10a10-flow-cytometry.jpg)
Flow Cytometry - Anti-Acrolein antibody [10A10] (ab240918)Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Acrolein using ab240918 at 1/50 dilution for 30 minutes on ice (solid line) compared to a non-specific IgG control (dashed line). Secondary antibody was a PE-conjugated goat anti-mouse IgG at 1/100 dilution for 20 minutes at room temperature. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
-
![Western blot - Anti-Acrolein antibody [10A10] (ab240918)](https://www.abcam.com/ps/products/240/ab240918/Images/ab240918-319765-anti-acrolein-antibody-10a10-western-blot.jpg)
Western blot - Anti-Acrolein antibody [10A10] (ab240918)All lanes : Anti-Acrolein antibody [10A10] (ab240918) at 1/1000 dilution
Lane 1 : Acrolein-BSA at 0.5 µg
Lane 2 : Acrolein-BSA at 2 µg
Lane 3 : BSA at 0.5 µg
Lane 4 : BSA at 2 µg
Secondary
All lanes : HRP-linked goat anti-mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlock: 5% Skim Milk in TBST.
Primary incubation for 2 hours at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab240918 has been referenced in 2 publications.
- Cui W et al. Acrolein Induces Systemic Coagulopathy via Autophagy-dependent Secretion of von Willebrand Factor in Mice after Traumatic Brain Injury. Neurosci Bull 37:1160-1175 (2021). PubMed: 33939120
- Wu X et al. Acrolein Aggravates Secondary Brain Injury After Intracerebral Hemorrhage Through Drp1-Mediated Mitochondrial Oxidative Damage in Mice. Neurosci Bull 36:1158-1170 (2020). PubMed: 32436179