Overview

  • Product name
    Anti-Acrolein antibody [5F6]
    See all Acrolein primary antibodies
  • Description
    Mouse monoclonal [5F6] to Acrolein
  • Host species
    Mouse
  • Specificity
    This antibody is specific for Acrolein modified protein (especially FDP-lysine type derivative).
  • Tested applications
    Suitable for: IHC-Pmore details
  • Immunogen

    Acrolein modified keyhole-lympet hemocyanine

  • Positive control
    • Aortic wall in the renal tissue of diabetic nephropathy or aortic wall of atherosclerosis.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab48501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Relevance
    Acrolein, a reactive alpha,beta-unsaturated aldehyde, is a common environmental pollutant, a metabolite of the anticancer drug cyclophosphamide, and a byproduct of lipid peroxidation. An increase in acrolein production has been proposed as a marker for Alzheimer's disease, diabetic glomerular lesions, and atherosclerosis. Acrolein is a potent inhibitor of cell proliferation at nonlethal doses and may act through effects on redox-regulated transcription factors. It has been reported that NF-kappaB activation is inhibited by acrolein.
  • Alternative names
    • 2-Propen-1-one antibody
    • 2-propenal antibody
    • Acraldehyde antibody
    • Acrolein antibody
    • Acrylic aldehyde antibody
    see all

Images

  • Immunohiostochemical analysis of Acrolein modified proteins expression in formalin fixed paraffin embedded atherosclerotic aorta using ab48501 at 0.5 µg/ml.

References

This product has been referenced in:
  • Aldahmash BA  et al. Attenuation of hepatotoxicity and oxidative stress in diabetes STZ-induced type 1 by biotin in Swiss albino mice. Saudi J Biol Sci 23:311-7 (2016). Read more (PubMed: 26981014) »
  • Chen D  et al. Cigarette smoke component acrolein modulates chromatin assembly by inhibiting histone acetylation. J Biol Chem 288:21678-87 (2013). Read more (PubMed: 23770671) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma, SHSY5Y)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Treatment
acrolein 24hr
Specification
neuroblastoma, SHSY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Jin Hui Liu

Verified customer

Submitted Jul 25 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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