Overview

  • Product name

    Anti-ACSL1 antibody [EPR13498]
    See all ACSL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13498] to ACSL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ACSL1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P33121

  • Positive control

    • Human fetal liver, heart and kidney lysates; HepG2 cell lysates; Human heart, kidney and liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 78 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
    • Tissue specificity

      Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
    • Sequence similarities

      Belongs to the ATP-dependent AMP-binding enzyme family.
    • Developmental stage

      Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
    • Cellular localization

      Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACS 1 antibody
      • ACS1 antibody
      • ACSL 1 antibody
      • ACSL1 antibody
      • ACSL1_HUMAN antibody
      • Acyl CoA synthetase 1 antibody
      • Acyl CoA synthetase long chain family member 1 antibody
      • Acyl-CoA synthetase 1 antibody
      • FACL 1 antibody
      • FACL 2 antibody
      • FACL1 antibody
      • FACL2 antibody
      • Fatty acid Coenzyme A ligase long chain 1 antibody
      • Fatty acid Coenzyme A ligase long chain 2 antibody
      • LACS 1 antibody
      • LACS 2 antibody
      • LACS antibody
      • LACS1 antibody
      • LACS2 antibody
      • Lignoceroyl CoA synthase antibody
      • Long chain acyl CoA synthetase 1 antibody
      • Long chain acyl CoA synthetase 2 antibody
      • Long chain fatty acid CoA ligase 1 antibody
      • Long chain fatty acid CoA ligase 2 antibody
      • Long chain fatty acid coenzyme A ligase 1 antibody
      • Long-chain acyl-CoA synthetase 1 antibody
      • Long-chain acyl-CoA synthetase 2 antibody
      • Long-chain fatty acid-CoA ligase 2 antibody
      • Long-chain-fatty-acid--CoA ligase 1 antibody
      • Palmitoyl CoA ligase 1 antibody
      • Palmitoyl CoA ligase 2 antibody
      • Palmitoyl-CoA ligase 1 antibody
      • Palmitoyl-CoA ligase 2 antibody
      • Paltimoyl CoA ligase 1 antibody
      see all

    Images

    • All lanes : Anti-ACSL1 antibody [EPR13498] (ab178419) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 78 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACSL1 with ab178419 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACSL1 with ab178419 at 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACSL1 with ab178419 at 1/50 dilution

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Belkaid A  et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). Read more (PubMed: 28334272) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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