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  1. Link

    acsl1-antibody-epr13499-ab177958.pdf

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Signal Transduction Metabolism Lipid metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ACSL1 antibody [EPR13499] (ab177958)

  • Datasheet
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Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
  • Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
  • Anti-ACSL1 antibody [EPR13499] (ab177958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13499] to ACSL1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human ACSL1 protein (ab116839)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Knockout
Product image
Human ACSL1 knockout HeLa cell line (ab265555)

View more associated products

Overview

  • Product name

    Anti-ACSL1 antibody [EPR13499]
    See all ACSL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13499] to ACSL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa and Human fetal liver lysates; Human kidney and liver tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13499
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-ACSL1 antibody [EPR13499] - BSA and Azide free (ab250028)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ACSL1 knockout HeLa cell line (ab265555)
  • KO cell lysates

    • Human ACSL1 knockout HeLa cell lysate (ab257335)
  • Recombinant Protein

    • Recombinant Human ACSL1 protein (ab116839)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
  • Tissue specificity

    Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Developmental stage

    Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P33121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2180 Human
    • Entrez Gene: 14081 Mouse
    • Entrez Gene: 25288 Rat
    • Omim: 152425 Human
    • SwissProt: P33121 Human
    • SwissProt: P41216 Mouse
    • SwissProt: P18163 Rat
    • Unigene: 406678 Human
    • Unigene: 210323 Mouse
    • Unigene: 6215 Rat
    see all
  • Alternative names

    • ACS 1 antibody
    • ACS1 antibody
    • ACSL 1 antibody
    • ACSL1 antibody
    • ACSL1_HUMAN antibody
    • Acyl CoA synthetase 1 antibody
    • Acyl CoA synthetase long chain family member 1 antibody
    • Acyl-CoA synthetase 1 antibody
    • FACL 1 antibody
    • FACL 2 antibody
    • FACL1 antibody
    • FACL2 antibody
    • Fatty acid Coenzyme A ligase long chain 1 antibody
    • Fatty acid Coenzyme A ligase long chain 2 antibody
    • LACS 1 antibody
    • LACS 2 antibody
    • LACS antibody
    • LACS1 antibody
    • LACS2 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 1 antibody
    • Long chain acyl CoA synthetase 2 antibody
    • Long chain fatty acid CoA ligase 1 antibody
    • Long chain fatty acid CoA ligase 2 antibody
    • Long chain fatty acid coenzyme A ligase 1 antibody
    • Long-chain acyl-CoA synthetase 1 antibody
    • Long-chain acyl-CoA synthetase 2 antibody
    • Long-chain fatty acid-CoA ligase 2 antibody
    • Long-chain-fatty-acid--CoA ligase 1 antibody
    • Palmitoyl CoA ligase 1 antibody
    • Palmitoyl CoA ligase 2 antibody
    • Palmitoyl-CoA ligase 1 antibody
    • Palmitoyl-CoA ligase 2 antibody
    • Paltimoyl CoA ligase 1 antibody
    see all

Images

  • Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ACSL1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab177958 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab177958 Anti-ACSL1 antibody [EPR13499] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab177958 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

    Immunohistochemical analysis of Human liver tissue labeling ACSL1 using ab177958 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

    Immunohistochemical analysis of Human kidney tissue labeling ACSL1 using ab177958 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa

  • Anti-ACSL1 antibody [EPR13499] (ab177958)
    Anti-ACSL1 antibody [EPR13499] (ab177958)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (8)

Publishing research using ab177958? Please let us know so that we can cite the reference in this datasheet.

ab177958 has been referenced in 8 publications.

  • Han J  et al. Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs. PLoS One 16:e0246279 (2021). PubMed: 33529214
  • Guo J  et al. Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Sepsis-Induced Acute Kidney Injury by Promoting Mitophagy of Renal Tubular Epithelial Cells via the SIRT1/Parkin Axis. Front Endocrinol (Lausanne) 12:639165 (2021). PubMed: 34248837
  • Luo Z  et al. Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity. J Cell Mol Med 25:8863-8876 (2021). PubMed: 34402182
  • Li T  et al. ACSL1 affects Triglyceride Levels through the PPAR? Pathway. Int J Med Sci 17:720-727 (2020). PubMed: 32218693
  • Wu H  et al. MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1a/CEBPB axis to promote stemness and chemo-resistance of gastric cancer. Cell Death Dis 11:233 (2020). PubMed: 32300102
  • Che L  et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
  • Yang L  et al. High expression of long chain acyl-coenzyme A synthetase 1 in peripheral blood may be a molecular marker for assessing the risk of acute myocardial infarction. Exp Ther Med 14:4065-4072 (2017). PubMed: 29104625
  • Huang C  et al. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules 21:N/A (2016). PubMed: 27153053

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