Recombinant Anti-ACSL1 antibody [EPR13499] (ab177958)
![Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)](https://www.abcam.com/ps/products/177/ab177958/Images/ab177958-391314-anti-acsl1-antibody-epr13499-ab177958-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13499] to ACSL1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ACSL1 antibody [EPR13499]
See all ACSL1 primary antibodies -
Description
Rabbit monoclonal [EPR13499] to ACSL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, HeLa and Human fetal liver lysates; Human kidney and liver tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13499 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
|
| Notes |
|---|
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 78 kDa. |
Target
-
Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. -
Tissue specificity
Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Developmental stage
Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2180 Human
- Entrez Gene: 14081 Mouse
- Entrez Gene: 25288 Rat
- Omim: 152425 Human
- SwissProt: P33121 Human
- SwissProt: P41216 Mouse
- SwissProt: P18163 Rat
- Unigene: 406678 Human
see all -
Alternative names
- ACS 1 antibody
- ACS1 antibody
- ACSL 1 antibody
see all
Images
-
Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ACSL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-3: Merged signal (red and green). Green - ab177958 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab177958 Anti-ACSL1 antibody [EPR13499] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab177958 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)](https://www.abcam.com/ps/products/177/ab177958/Images/ab177958-205991-anti-acsl1-antibody-epr13499-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)Immunohistochemical analysis of Human liver tissue labeling ACSL1 using ab177958 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)](https://www.abcam.com/ps/products/177/ab177958/Images/ab177958-205990-anti-acsl1-antibody-epr13499-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)Immunohistochemical analysis of Human kidney tissue labeling ACSL1 using ab177958 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)](https://www.abcam.com/ps/products/177/ab177958/Images/ab177958-205989-anti-acsl1-antibody-epr13499-western-blot.jpg)
Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa -
![Anti-ACSL1 antibody [EPR13499] (ab177958)](https://www.abcam.com/ps/products/177/ab177958/Images/ab177958-6-benefits-of-recombinant-antibodies.png)
Anti-ACSL1 antibody [EPR13499] (ab177958)
Protocols
Datasheets and documents
-
Datasheet download
References (5)
ab177958 has been referenced in 5 publications.
- Li T et al. ACSL1 affects Triglyceride Levels through the PPAR? Pathway. Int J Med Sci 17:720-727 (2020). PubMed: 32218693
- Wu H et al. MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1a/CEBPB axis to promote stemness and chemo-resistance of gastric cancer. Cell Death Dis 11:233 (2020). PubMed: 32300102
- Che L et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). PubMed: 30985894
- Yang L et al. High expression of long chain acyl-coenzyme A synthetase 1 in peripheral blood may be a molecular marker for assessing the risk of acute myocardial infarction. Exp Ther Med 14:4065-4072 (2017). PubMed: 29104625
- Huang C et al. Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats. Molecules 21:N/A (2016). PubMed: 27153053
