Overview

  • Product name
    Anti-ACSL3 antibody
    See all ACSL3 primary antibodies
  • Description
    Rabbit polyclonal to ACSL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 416-637 of Human ACSL3 (O95573).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab151959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
  • Sequence similarities
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization
    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACS3 antibody
    • Acsl3 antibody
    • ACSL3_HUMAN antibody
    • Acyl CoA synthetase long chain family member 3 antibody
    • FACL3 antibody
    • Fatty acid Coenzyme A ligase long chain 3 antibody
    • LACS 3 antibody
    • LACS3 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 3 antibody
    • Long chain fatty acid CoA ligase 3 antibody
    • Long-chain acyl-CoA synthetase 3 antibody
    • Long-chain-fatty-acid--CoA ligase 3 antibody
    • PRO2194 antibody
    see all

Images

  • Western blot - Anti-ACSL3 antibody (ab151959)
    Western blot - Anti-ACSL3 antibody (ab151959)
    All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)
    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.

References

ab151959 has not yet been referenced specifically in any publications.

Publishing research using ab151959? Please let us know so that we can cite the reference in this datasheet.

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