Anti-ACSL3 antibody (ab151959)

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Western blot - Anti-ACSL3 antibody (ab151959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)

Key features and details

  • Rabbit polyclonal to ACSL3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ACSL3 antibody

  • Description

    Rabbit polyclonal to ACSL3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 416-637 of Human ACSL3 (O95573).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • 293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Pig
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).

  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.

  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACS3 antibody
    • Acsl3 antibody
    • ACSL3_HUMAN antibody
    • Acyl CoA synthetase long chain family member 3 antibody
    • FACL3 antibody
    • Fatty acid Coenzyme A ligase long chain 3 antibody
    • LACS 3 antibody
    • LACS3 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 3 antibody
    • Long chain fatty acid CoA ligase 3 antibody
    • Long-chain acyl-CoA synthetase 3 antibody
    • Long-chain-fatty-acid--CoA ligase 3 antibody
    • PRO2194 antibody
    see all

Images

  • Western blot - Anti-ACSL3 antibody (ab151959)
    Western blot - Anti-ACSL3 antibody (ab151959)
    All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)
    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.

References (2)

Publishing research using ab151959? Please let us know so that we can cite the reference in this datasheet.

ab151959 has been referenced in 2 publications.

  • Kankeu C  et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). PubMed: 29770421
  • Belkaid A  et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). PubMed: 28334272

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