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Rabbit polyclonal to ACSL3
Predicted to work with:
Mouse, Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 416-637 of Human ACSL3 (O95573).
293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
Belongs to the ATP-dependent AMP-binding enzyme family.
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
Information by UniProt
Western blot - Anti-ACSL3 antibody (ab151959)
All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 80 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"