Anti-ACSL3 antibody (ab151959)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-ACSL3 antibody
See all ACSL3 primary antibodies -
Description
Rabbit polyclonal to ACSL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 416-637 of Human ACSL3 (O95573).
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Positive control
- 293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2181 Human
- Entrez Gene: 74205 Mouse
- Entrez Gene: 100233169 Pig
- Entrez Gene: 114024 Rat
- Omim: 602371 Human
- SwissProt: O95573 Human
- SwissProt: Q9CZW4 Mouse
- SwissProt: Q63151 Rat
see all -
Alternative names
- ACS3 antibody
- Acsl3 antibody
- ACSL3_HUMAN antibody
see all
Images
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Western blot - Anti-ACSL3 antibody (ab151959)All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.
References
This product has been referenced in:
- Kankeu C et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). Read more (PubMed: 29770421) »
- Belkaid A et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). Read more (PubMed: 28334272) »
