Product nameAnti-ACSL3 antibody
See all ACSL3 primary antibodies
DescriptionRabbit polyclonal to ACSL3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
- 293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 80 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAcyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- ACS3 antibody
- Acsl3 antibody
- ACSL3_HUMAN antibody
All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.
This product has been referenced in:
- Kankeu C et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). Read more (PubMed: 29770421) »
- Belkaid A et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). Read more (PubMed: 28334272) »