Product nameAnti-ACSL3 antibody
See all ACSL3 primary antibodies
DescriptionRabbit polyclonal to ACSL3
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Orangutan
- 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab80675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionAcyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- ACS3 antibody
- Acsl3 antibody
- ACSL3_HUMAN antibody
Anti-ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Application: IHC. Species+tissue/cell type:THP-1 derived macrophage activated with iMtb. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333
Application: IHC. Species+tissue/cell type:THP-1 derived macrophage. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333