Key features and details
- Rabbit polyclonal to ACSL6/ACS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ACSL6/ACS2 antibody
See all ACSL6/ACS2 primary antibodies
DescriptionRabbit polyclonal to ACSL6/ACS2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ACSL6/ACS2 aa 458-697.
Database link: Q9UKU0
- WB: Mouse liver, heart, brain and gonadal lysates; Jurkat, Raji and K562 whole cell lysates. IHC-P: Human brain and adrenal gland tissues.
This product was previously labelled as ACSL6
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Mouse liver tissue lysate - total protein (ab29301)
- Raji whole cell lysate (ab30124)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (ab4022)
- Jurkat whole cell lysate (ab7899)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab229958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
RelevanceACSL6 belongs to the ATP-dependent AMP-binding enzyme family. It is involved in the activation of long-chain fatty acids for both synthesis of cellular lipids and degradation via beta-oxidation. ACSL6 plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid. ACSL6 is expressed predominantly in erythrocyte precursors, in particular in reticulocytes, fetal blood cells derived from fetal liver, haemopoietic stem cells from cord blood, bone marrow, and brain. There are three named isoforms.
Cellular localizationMitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.
- ACS2 antibody
- Acyl CoA synthetase long chain family member 6 antibody
- FACL6 antibody
All lanes : Anti-ACSL6/ACS2 antibody (ab229958) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse gonadal lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Paraffin-embedded human brain tissue stained for ACSL6/ACS2 using ab229958 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for ACSL6/ACS2 using ab229958 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229958 has not yet been referenced specifically in any publications.