Key features and details
- Rabbit polyclonal to ACSL6/ACS2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACSL6/ACS2 antibody
See all ACSL6/ACS2 primary antibodies
DescriptionRabbit polyclonal to ACSL6/ACS2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human ACSL6/ACS2 aa 499-548. The exact sequence is proprietary.
Database link: Q9UKU0
- WB: COLO 205 and Jurkat cell extracts. IHC-P: Human skeletal muscle tissue.
This product was previously labelled as ACSL6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Mg2+ and Ca2+ free.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 78 kDa.|
RelevanceACSL6 belongs to the ATP-dependent AMP-binding enzyme family. It is involved in the activation of long-chain fatty acids for both synthesis of cellular lipids and degradation via beta-oxidation. ACSL6 plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid. ACSL6 is expressed predominantly in erythrocyte precursors, in particular in reticulocytes, fetal blood cells derived from fetal liver, haemopoietic stem cells from cord blood, bone marrow, and brain. There are three named isoforms.
Cellular localizationMitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein.
- ACS2 antibody
- Acyl CoA synthetase long chain family member 6 antibody
- FACL6 antibody
All lanes : Anti-ACSL6/ACS2 antibody (ab230415) at 1/500 dilution
Lane 1 : COLO 205 (human colon adenocarcinoma cell line) cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 3 : COLO 205 cell extract with immunizing peptide
Predicted band size: 78 kDa
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ACSL6/ACS2 using ab230415 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230415 has not yet been referenced specifically in any publications.