Key features and details
- Rabbit polyclonal to ACSS1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACSS1 antibody
DescriptionRabbit polyclonal to ACSS1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: Human cell line RPMI-8226 IHC-P: Human placenta tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 74 kDa.|
FunctionImportant for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
modificationsReversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- ACAS2L antibody
- AceCS2 antibody
- AceCS2L antibody
Anti-ACSS1 antibody (ab224176) at 1/250 dilution + Human cell line RPMI-8226
Predicted band size: 74 kDa
ab224176 staining ACSS1 of human paraffin-embedded liver tissue by immunohistochemcial analysis shows no positivity in hepatocytes as expected.
ab224176 staining ACSS1 of human paraffin-embedded placenta tissue by immunohistochemcial analysis shows strong granular cytoplasmic positivity in trophoblastic cells.
ab224176 staining ACSS1 of human paraffin-embedded upper duodenum tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in glandular cells.
ab224176 staining ACSS1 of human paraffin-embedded testis tissue by immunohistochemcial analysis shows moderate granular cytoplasmic positivity in Leydig cells.
ab224176 has not yet been referenced specifically in any publications.