Overview

  • Product name

    Anti-ACSS2 antibody [EPR8500]
    See all ACSS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8500] to ACSS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ACSS2 aa 100-200.
    Database link: Q9NR19

  • Positive control

    • WB: U-87 MG, HepG2, Caco 2 and fetal liver lysates, Mouse and Rat brain lysate; ICC/IF: Caco 2 and HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Activates acetate so that it can be used for lipid synthesis or for energy generation.
    • Sequence similarities

      Belongs to the ATP-dependent AMP-binding enzyme family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAS2 antibody
      • AceCS antibody
      • Acetate CoA ligase antibody
      • Acetate thiokinase antibody
      • Acetate--CoA ligase antibody
      • Acetyl CoA synthetase antibody
      • Acetyl Coenzyme A synthetase 2 (ADP forming) antibody
      • Acetyl coenzyme A synthetase cytoplasmic antibody
      • Acetyl-CoA synthetase antibody
      • Acetyl-coenzyme A synthetase antibody
      • ACS antibody
      • ACSA antibody
      • ACSA_HUMAN antibody
      • ACSS2 antibody
      • Acyl activating enzyme antibody
      • Acyl CoA synthetase short chain family member 2 antibody
      • Acyl-activating enzyme antibody
      • Acyl-CoA synthetase short-chain family member 2 antibody
      • Cytoplasmic acetyl coenzyme A synthetase antibody
      • cytoplasmic antibody
      • MYH7B antibody
      see all

    Images

    • All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Purified)

      Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 79 kDa
      Observed band size: 78 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ACSS2 with purified ab133664 at 1:200 dilution (8.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Unpurified)

      Lane 1 : U87 MG lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Caco 2 lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 79 kDa

    • Immunofluorescence analysis of ACSS2 in Caco 2 cells labelled with unpurified ab133664 at a 1/100 dilution.

    References

    This product has been referenced in:

    • Gao X  et al. Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia. Nat Commun 7:11960 (2016). IHC . Read more (PubMed: 27357947) »
    • Mahmood S  et al. Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway. Am J Physiol Endocrinol Metab 311:E117-27 (2016). Read more (PubMed: 27166281) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    60 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C

    Miss. Raquel Jurado-López

    Verified customer

    Submitted Feb 27 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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