Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8500] to ACSS2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-ACSS2 antibody [EPR8500]
See all ACSS2 primary antibodies
DescriptionRabbit monoclonal [EPR8500] to ACSS2
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human ACSS2 aa 100-200.
Database link: Q9NR19
- WB: U-87 MG, HepG2, Caco 2 and fetal liver lysates, Mouse and Rat brain lysate; ICC/IF: Caco 2 and HepG2 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 79 kDa.|
FunctionActivates acetate so that it can be used for lipid synthesis or for energy generation.
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
- Information by UniProt
- ACAS2 antibody
- AceCS antibody
- Acetate CoA ligase antibody
All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ACSS2 with purified ab133664 at 1:200 dilution (8.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Unpurified)
Lane 1 : U87 MG lysate
Lane 2 : HepG2 lysate
Lane 3 : Caco 2 lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa
Immunofluorescence analysis of ACSS2 in Caco 2 cells labelled with unpurified ab133664 at a 1/100 dilution.
ab133664 has been referenced in 4 publications.
- He W et al. MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer. Oncogene 38:4637-4654 (2019). PubMed: 30742067
- Gao X et al. Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia. Nat Commun 7:11960 (2016). IHC . PubMed: 27357947
- Mahmood S et al. Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway. Am J Physiol Endocrinol Metab 311:E117-27 (2016). PubMed: 27166281
- Soliman ML et al. Acetate reduces microglia inflammatory signaling in vitro. J Neurochem 123:555-67 (2012). WB ; Mouse . PubMed: 22924711