Recombinant
RabMAb

Recombinant Anti-ACSS2 antibody [EPR8500] - BSA and Azide free (ab240077)

Overview

  • Product name

    Anti-ACSS2 antibody [EPR8500] - BSA and Azide free
    See all ACSS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8500] to ACSS2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACSS2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: U-87 MG, HepG2, Caco 2 and fetal liver lysates, Mouse and Rat brain lysate; ICC/IF: Caco 2 and HepG2 cells
  • General notes

    Ab240077 is the carrier-free version of ab133664. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240077 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Activates acetate so that it can be used for lipid synthesis or for energy generation.
    • Sequence similarities

      Belongs to the ATP-dependent AMP-binding enzyme family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAS2 antibody
      • AceCS antibody
      • Acetate CoA ligase antibody
      • Acetate thiokinase antibody
      • Acetate--CoA ligase antibody
      • Acetyl CoA synthetase antibody
      • Acetyl Coenzyme A synthetase 2 (ADP forming) antibody
      • Acetyl coenzyme A synthetase cytoplasmic antibody
      • Acetyl-CoA synthetase antibody
      • Acetyl-coenzyme A synthetase antibody
      • ACS antibody
      • ACSA antibody
      • ACSA_HUMAN antibody
      • ACSS2 antibody
      • Acyl activating enzyme antibody
      • Acyl CoA synthetase short chain family member 2 antibody
      • Acyl-activating enzyme antibody
      • Acyl-CoA synthetase short-chain family member 2 antibody
      • Cytoplasmic acetyl coenzyme A synthetase antibody
      • cytoplasmic antibody
      • MYH7B antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ACSS2 with purified ab133664 at 1:200 dilution (8.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide�(ab133664)
    • Immunofluorescence analysis of ACSS2 in Caco 2 cells labelled with unpurified ab133664 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133664).

    References

    ab240077 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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