Key features and details
- Rabbit polyclonal to ACT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ACT antibody
DescriptionRabbit polyclonal to ACT
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant protein fragment corresponding to a region within amino acids 1 and 196 of human ACT (NP_065228)
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cells. SW480 xenograft.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionMay be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner.
Tissue specificityTestis-specific (at protein level).
Sequence similaritiesContains 4 LIM zinc-binding domains.
Cellular localizationNucleus. Nuclei of round and elongated spermatids.
- Information by UniProt
- Activator of cAMP responsive element modulator (CREM) in testis antibody
- Activator of cAMP-responsive element modulator in testis antibody
- Activator of CREM in testis antibody
All lanes : Anti-ACT antibody (ab97396) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
Gel conc 12 % (SDS PAGE)
ab97396 at 1/500 dilution, staining human ACT in SW480 xenograft - Formalin/PFA-fixed paraffin-embedded tissue.
ab97396 has not yet been referenced specifically in any publications.