Key features and details
- Mouse monoclonal [C3] to ACTC1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-ACTC1 antibody [C3]
See all ACTC1 primary antibodies
DescriptionMouse monoclonal [C3] to ACTC1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Human, Xenopus tropicalis
Recombinant fragment (His-T7-tag) corresponding to Human ACTC1 aa 156-368. N-terminal tags. Expressed in E.coli.
DSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTT AEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNE RFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGT TMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQ QMWISKQEYDEAG
Database link: P68032
- WB: Recombinant human ACTC1 protein; Mouse heart, skeletal muscle and skin lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:613424]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in ACTC1 are the cause of atrial septal defect type 5 (ASD5) [MIM:612794]. A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ACTC antibody
- ACTC_HUMAN antibody
- ACTC1 antibody
Anti-ACTC1 antibody [C3] (ab239475) at 3 µg/ml + Recombinant human ACTC1 protein
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 42 kDa
All lanes : Anti-ACTC1 antibody [C3] (ab239475) at 3 µg/ml
Lane 1 : Mouse heart lysate
Lane 2 : Mouse skeletal muscle lysate
Lane 3 : Mouse skin lysate
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239475 has not yet been referenced specifically in any publications.