Overview

  • Product name

  • Description

    Rabbit polyclonal to ACTG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Pig
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Recombinant full length protein corresponding to Human ACTG2 aa 3-376. (Expressed in E.coli).
    Database link: P63267

  • Positive control

    • IHC-P: Human lung, liver, prostate, stomach, prostate cancer and colorectal cancer tissues. WB: Human lung lysate; Pig and rat stomach lysates; Recombinant human ACTG2 protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab231802 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
    Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACT antibody
    • ACTA3 antibody
    • ACTE antibody
    • ACTG2 antibody
    • ACTH_HUMAN antibody
    • Actin antibody
    • Actin gamma 2 smooth muscle enteric antibody
    • Actin gamma enteric smooth muscle antibody
    • Actin like protein antibody
    • ACTL3 antibody
    • ACTSG antibody
    • Alpha actin 3 antibody
    • Alpha-actin-3 antibody
    • Gamma 2 actin antibody
    • Gamma-2-actin antibody
    • gamma-enteric smooth muscle antibody
    • Smooth muscle gamma actin antibody
    • Smooth muscle gamma-actin antibody
    see all

Images

  • Paraffin-embedded human lung tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human liver tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human prostate tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human stomach tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human prostate cancer tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human colorectal cancer tissue stained for ACTG2 using ab231802 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Anti-ACTG2 antibody (ab231802) at 2 µg/ml + Recombinant human ACTG2 protein

    Secondary
    HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

  • All lanes : Anti-ACTG2 antibody (ab231802) at 2 µg/ml

    Lane 1 : Human lung lysate
    Lane 2 : Pig stomach lysate
    Lane 3 : Rat stomach lysate

    Secondary
    All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

References

ab231802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab231802.
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