Key features and details
- Rabbit polyclonal to ACTG2 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ACTG2 antibody - N-terminal
See all ACTG2 primary antibodies
DescriptionRabbit polyclonal to ACTG2 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human uterus and prostate tissue. Extracts from COLO205 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+, Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 42 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Sequence similaritiesBelongs to the actin family.
modificationsOxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- ACT antibody
- ACTA3 antibody
- ACTE antibody
All lanes : Anti-ACTG2 antibody - N-terminal (ab189385) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells
Lane 2 : Extracts from COLO205 cells treated with the synthesized peptide
Predicted band size: 42 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue sections labeling ACTG2 using ab189385 at 5 µg/ml.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human uterus tissue sections labeling ACTG2 using ab189385 at 5 µg/ml.
ab189385 has not yet been referenced specifically in any publications.