Recombinant Anti-ACTH antibody [57] - N-terminal- BSA and Azide free (ab212736)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [57] to ACTH - N-terminal – BSA and Azide free
- Suitable for: IHC-P, mIHC
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-ACTH antibody [57] - N-terminal- BSA and Azide free
See all ACTH primary antibodies -
Description
Mouse monoclonal [57] to ACTH - N-terminal – BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, mIHCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Guinea pig, Cow, a wide range of other species, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human ACTH (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: P01189 -
Positive control
- IHC: Human, mouse, rat pituitary gland tissues. mIHC: Human pituitary gland.
-
General notes
This product was switched from a hybridoma to recombinant production format on 25th November 2021.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
57 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species |
|
mIHC |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species |
mIHC
Use at an assay dependent concentration. |
Target
-
Relevance
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 5443 Human
- Entrez Gene: 694430 Macaque monkey
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Entrez Gene: 443212 Sheep
- Omim: 176830 Human
- SwissProt: P01190 Cow
- SwissProt: P19402 Guinea pig
see all -
Alternative names
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
Images
-
This data was developed using ab233954, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pituitary gland labelling ACTH with ab233954 at 1/5000 dilution (0.214 μg/ml) (B), Luteinizing Hormone beta with ab221494 at 1/4000 dilution (0.258 μg/ml) (C) and Growth Hormone with ab155276 at 1/5000 dilution (0.044 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Growth Hormone (gray; Opal™690), anti-ACTH (green; Opal™520) and anti-Luteinizing Hormone beta (red; Opal™570) on human pituitary gland.
Panel B: anti-ACTH stained on corticotrophs.
Panel C: anti-Luteinizing Hormone beta stained on gonadotrophs.
Panel D: anti-Growth Hormone stained on somatotrophs.The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab221494 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
-
Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (ab233954; green; Opal™520) at 1:5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1:500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (ab155276; gray; Opal™690) at 1:5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
This data was developed using ab233954, the same antibody clone in a different buffer formulation.
-
This data was developed using ab233954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Human pituatary gland tissue stained for ACTH using ab233954 at 1/5000 dilution. Positive staining on human pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
This data was developed using ab233954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Mouse pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on mouse pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
-
This data was developed using ab233954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Rat pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on ratpituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Datasheets and documents
-
Datasheet download
References (0)
ab212736 has not yet been referenced specifically in any publications.