• Product name
  • Description
    Rabbit polyclonal to ACTH
  • Host species
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Goat, Chicken, Guinea pig, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to ACTH aa 1-39. This corresponds to amino acids 138-176 of the precursor POMC.


    Database link: P01189

  • Positive control
    • IHC-P: P0 Pitx2-Cre/Dicer1 mutant mice pituitary tissue. Mouse brain tissue.



Our Abpromise guarantee covers the use of ab74976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 29 kDa.
IP 1/200.


  • Relevance
    ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
  • Cellular localization
  • Database links
  • Alternative names
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • Alpha melanocyte stimulating hormone antibody
    • alpha-MSH antibody
    • alphaMSH antibody
    • Beta LPH antibody
    • Beta melanocyte stimulating hormone antibody
    • Beta-endorphin antibody
    • beta-MSH antibody
    • CLIP antibody
    • Corticotropin antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-like intermediary peptide antibody
    • Gamma LPH antibody
    • gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • Lipotropin, included antibody
    • LPH antibody
    • Melanocyte-stimulating hormone, included antibody
    • Melanotropin alpha antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • Melanotropin, included antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • Opiomelanocortin prepropeptide antibody
    • POC antibody
    • POMC antibody
    • Pomc-1 antibody
    • Pomc1 antibody
    • Pomc2 antibody
    • Pro ACTH endorphin antibody
    • Pro opiomelanocortin antibody
    • Pro-opiomelanocortin-alpha antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    • Tetracosactide antibody
    see all


  • Formalin-fixed, paraffin-embedded mouse brain tissue stained for ACTH with ab74976 (1/100 dilution) in immunohistochemical analysis. DAB (brown) and Hematoxylin (blue)staining.

  • ab74976 staining ACTH in P0 Pitx2-Cre/Dicer1 mutant mice pituitary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Samples were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin wax. Sections were cut (7 µm) and stained with H&E. Immunohistochemistry was performed on 7-µm paraffin sections stained by an indirect immunoperoxidase method. Peroxidase activity was visualized with AEC stain kit. ab74976 diluted 1/200. Secondary antibodies conjugated with biotin were used at 1/150 dilution.


This product has been referenced in:
  • Pu J  et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett 12:585-590 (2016). IHC-P ; Human . Read more (PubMed: 27347184) »
  • Li WY  et al. Paraneoplastic Cushing's syndrome associated with bronchopulmonary carcinoid tumor in youth: A case report and review of the literature. Oncol Lett 12:69-72 (2016). IHC-P ; Human . Read more (PubMed: 27347101) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Pig Purified protein (brain)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 27 2015


Thank you for contacting us.

Unfortunately we do not have any images for sharing we however can provide 100% image discount, if customer tests the antibody and submit an image in the form of Abreview. Please let me know if you want an Abtrial code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

You can use any antibody however we will not be able to provide details about the purity of isolated protein.

The antibody ab2407 is in whole antiserum which means the concentration is not available so the optimization of the IP dilution would be necessary for better results.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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