Key features and details
- Mouse monoclonal [AH26 + 57] to ACTH - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ACTH antibody [AH26 + 57] - BSA and Azide free
See all ACTH primary antibodies
DescriptionMouse monoclonal [AH26 + 57] to ACTH - BSA and Azide free
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human ACTH aa 1-24 conjugated to keyhole limpet haemocyanin. This sequence corresponds to amino acids 138-162 of the precursor POMC. (AH26).
Database link: P01189
Recombinant fragment corresponding to Human ACTH (N terminal) conjugated to keyhole limpet haemocyanin. (57).
Database link: P01189
- IHC-P: Human pituitary gland tissue.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesab212738 was purified from Bioreactor Concentrate by Protein A/G.
Clone numberAH26 + 57
Light chain typekappa
Our Abpromise guarantee covers the use of ab212738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
RelevanceACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
Formalin-fixed, paraffin-embedded human pituitary gland tissue stained for ACTH using 212738 at 1 µg/ml in immunohistochemical analysis.
This IHC-P data was generated using the same anti-ACTH antibody clone, AH26 + 57, in a different buffer formulation (ab199007).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab212738 has not yet been referenced specifically in any publications.