Recombinant Anti-ACTH antibody [AH26] (ab233953)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AH26] to ACTH
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ACTH antibody [AH26]
See all ACTH primary antibodies -
Description
Mouse monoclonal [AH26] to ACTH -
Host species
Mouse -
Specificity
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This antibody is specific to Synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH).
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, Mouse and Rat pituitary gland tissue. IHC-Fr: Mouse pituitary gland tissue
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General notes
This product has switched from a hybridoma to recombinant production method on 11th March 2022.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AH26 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/100.
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IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-Fr
1/100. |
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 5443 Human
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Omim: 176830 Human
- SwissProt: P01189 Human
- SwissProt: P01193 Mouse
- SwissProt: P01194 Rat
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Alternative names
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human pituitary gland tissue labeling ACTH with ab233953 at 1/5000 (0.233 µg/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Positive staining on human pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1:1000) for 8mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1:1000) for 8mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary (fresh frozen) tissue labeling ACTH with ab233953 at 1/100 (11.64 µg/ml) dilution followed by ab150113, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green).
Positive staining on mouse pituitary. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233953 has not yet been referenced specifically in any publications.