Key features and details
- Mouse monoclonal [5J11] to Actin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Horse, Chicken, Cow, Human, Pig
- Isotype: IgG1
Product nameAnti-Actin antibody [5J11]
See all Actin primary antibodies
DescriptionMouse monoclonal [5J11] to Actin
Specificityab190301 binds to all six isotypes of mammalian actin.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Horse, Chicken, Cow, Human, Pig
Predicted to work with: Rabbit, Orangutan
Full length native protein (purified) corresponding to Cow Actin aa 3-377. derived from bovine brain preparation.
DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQK DSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG IVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSF VTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLS TFQQMWITKQEYDEAGPSIVHRKCF
Database link: P68138
- WB: Rat and mouse brain lysate; NIH/3T3, HEK-293, HeLa and SH-SY5Ycell lysate. ICC/IF: HeLa cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 49.98% PBS, 49.98% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab190301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/1000. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- a-actin antibody
- ACTA antibody
All lanes : Anti-Actin antibody [5J11] (ab190301) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 42 kDa
The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).
Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.
ab190301 has not yet been referenced specifically in any publications.