Overview

  • Product name
    Anti-Actin antibody [5J11]
    See all Actin primary antibodies
  • Description
    Mouse monoclonal [5J11] to Actin
  • Host species
    Mouse
  • Specificity
    ab190301 binds to all six isotypes of mammalian actin.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Chicken, Cow, Human, Pig
    Predicted to work with: Rabbit, Orangutan
  • Immunogen

    Full length native protein (purified) corresponding to Cow Actin aa 3-377. derived from bovine brain preparation.
    Sequence:

    DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQK DSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE HPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTG IVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSF VTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLS TFQQMWITKQEYDEAGPSIVHRKCF


    Database link: P68138

  • Positive control
    • WB: Rat and mouse brain lysate; NIH/3T3, HEK-293, HeLa and SH-SY5Ycell lysate. ICC/IF: HeLa cells.

Applications

Our Abpromise guarantee covers the use of ab190301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
WB 1/1000. Predicted molecular weight: 42 kDa.

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Images

  • All lanes : Anti-Actin antibody [5J11] (ab190301) at 1/1000 dilution

    Lane 1 : Protein standard
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 7 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Predicted band size: 42 kDa



    The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).

  • Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.

References

ab190301 has not yet been referenced specifically in any publications.

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