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  1. Link

    actin-antibody-ab197345.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-Actin antibody (ab197345)

  • Datasheet
Submit a review Submit a question References (7)

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Western blot - Anti-Actin antibody (ab197345)

    Key features and details

    • Rabbit polyclonal to Actin
    • Suitable for: WB
    • Reacts with: Arabidopsis thaliana, Plants
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Actin antibody
      See all Actin primary antibodies
    • Description

      Rabbit polyclonal to Actin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Arabidopsis thaliana, Plants
    • Immunogen

      Synthetic peptide corresponding to Arabidopsis thaliana Actin (N terminal). (NP_190236.1).
      Database link: P53497

    • Positive control

      • Cinnamomum camphora, Nicotiana tabacum, Triticum aestivuml, Arabidopsis thaliana, Brassica campestris, Vicit Faba lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.4
      Preservative: 0.05% Sodium azide
      Constituents: 50% Glycerol, 49% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Troponin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Tropomyosin
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Crosslinking
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Binding Proteins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Assembly
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Death receptors & ligands
      • RIP
      • Isotype/Loading Controls
      • Loading Controls
      • Actin
      • Cancer
      • Cell Death
      • Apoptosis
      • Receptors
      • Death receptors & ligands
      • RIP
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab197345 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 42 kDa.
    Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 42 kDa.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • a actin antibody
      • a-actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Images

    • Western blot - Anti-Actin antibody (ab197345)
      Western blot - Anti-Actin antibody (ab197345)
      All lanes : Anti-Actin antibody (ab197345) at 1/500 dilution

      Lane 1 : Cinnamomum camphora leaves lysate
      Lane 2 : Nicotiana tabacum leaves lysate
      Lane 3 : Triticum aestivuml leaves lysate
      Lane 4 : Arabidopsis thaliana leaves lysate
      Lane 5 : Brassica campestris flowers leaves lysate
      Lane 6 : Brassica campestris leaves lysate
      Lane 7 : Vicit Faba leaves lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG at 1/8000 dilution

      Developed using the ECL technique.

      Predicted band size: 42 kDa


      Exposure time: 20 seconds


      10%SDS-PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (7)

    Publishing research using ab197345? Please let us know so that we can cite the reference in this datasheet.

    ab197345 has been referenced in 7 publications.

    • Zhang T  et al. Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency. Int J Mol Sci 22:N/A (2021). PubMed: 34445480
    • Almoguera C  et al. Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening. Plants (Basel) 9:N/A (2020). PubMed: 32155706
    • Yang C  et al. HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation. Mol Plant 13:515-531 (2020). PubMed: 32087368
    • Wang Y  et al. BZR1 Mediates Brassinosteroid-Induced Autophagy and Nitrogen Starvation in Tomato. Plant Physiol 179:671-685 (2019). PubMed: 30482787
    • Lim SD  et al. A Vitis vinifera basic helix-loop-helix transcription factor enhances plant cell size, vegetative biomass, and reproductive yield. Plant Biotechnol J N/A:N/A (2018). WB . PubMed: 29520945
    • Ma J  et al. Histone chaperones play crucial roles in maintenance of stem cell niche during plant root development. Plant J 95:86-100 (2018). PubMed: 29676825
    • Wang P  et al. Reciprocal Regulation of the TOR Kinase and ABA Receptor Balances Plant Growth and Stress Response. Mol Cell 69:100-112.e6 (2018). PubMed: 29290610

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