Overview

  • Product name
  • Description
    Rabbit polyclonal to Actin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Plants
  • Immunogen

    Synthetic peptide corresponding to Arabidopsis thaliana Actin (N terminal). (NP_190236.1).
    Database link: P53497

  • Positive control
    • Cinnamomum camphora, Nicotiana tabacum, Triticum aestivuml, Arabidopsis thaliana, Brassica campestris, Vicit Faba lysates.

Applications

Our Abpromise guarantee covers the use of ab197345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.

Target

  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Images

  • All lanes : Anti-Actin antibody (ab197345) at 1/500 dilution

    Lane 1 : Cinnamomum camphora leaves lysate
    Lane 2 : Nicotiana tabacum leaves lysate
    Lane 3 : Triticum aestivuml leaves lysate
    Lane 4 : Arabidopsis thaliana leaves lysate
    Lane 5 : Brassica campestris flowers leaves lysate
    Lane 6 : Brassica campestris leaves lysate
    Lane 7 : Vicit Faba leaves lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG at 1/8000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa


    Exposure time: 20 seconds


    10%SDS-PAGE

References

This product has been referenced in:
  • Lim SD  et al. A Vitis vinifera basic helix-loop-helix transcription factor enhances plant cell size, vegetative biomass, and reproductive yield. Plant Biotechnol J N/A:N/A (2018). WB . Read more (PubMed: 29520945) »
  • Ma J  et al. Histone chaperones play crucial roles in maintenance of stem cell niche during plant root development. Plant J 95:86-100 (2018). Read more (PubMed: 29676825) »
See all 3 Publications for this product

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