Overview

  • Product name

  • Description

    Rabbit polyclonal to Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC - Wholemount, IHCmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish Actin aa 2-179. The exact sequence is proprietary.
    Database link: Q4KMI

  • Positive control

    • Whole zebrafish extract; 1 day-post-fertilization zebrafish embryo; zebrafish tissue; zebrafish embryo tail section.

Applications

Our Abpromise guarantee covers the use of ab209857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000.

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95C,1Hr

IHC - Wholemount 1/50 - 1/500.
IHC 1/100 - 1/1000.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • a-actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Images

  • Anti-Actin antibody (ab209857) at 1/1000 dilution + whole zebrafish extract at 30 µg

    Predicted band size: 42 kDa



    10% SDS-PAGE

  • Immunohistochemical analysis of agarose-embedded Zebrafish embryo tail section labeling Actin with ab209857 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling Actin with ab209857 at 1/300 dilution.

  • Whole mount immunohistochemical analysis of of 1 day-post-fertilization zebrafish embryo labeling Actin with ab209857 at 1/50 dilution.

References

ab209857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209857.
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