Key features and details
- Rabbit polyclonal to Actin
- Suitable for: WB, IHC-P, IHC - Wholemount, IHC
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-Actin antibody
See all Actin primary antibodies
DescriptionRabbit polyclonal to Actin
Tested applicationsSuitable for: WB, IHC-P, IHC - Wholemount, IHCmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment within Zebrafish Actin aa 2-179. The exact sequence is proprietary.
Database link: Q4KMI
- Whole zebrafish extract; 1 day-post-fertilization zebrafish embryo; zebrafish tissue; zebrafish embryo tail section.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab209857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/1000.
Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95C,1Hr
|IHC - Wholemount||1/50 - 1/500.|
|IHC||1/100 - 1/1000.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Entrez Gene: 407658 Zebrafish
- a actin antibody
- a-actin antibody
- ACTA antibody
Anti-Actin antibody (ab209857) at 1/1000 dilution + whole zebrafish extract at 30 µg
Predicted band size: 42 kDa
Immunohistochemical analysis of agarose-embedded Zebrafish embryo tail section labeling Actin with ab209857 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue labeling Actin with ab209857 at 1/300 dilution.
Whole mount immunohistochemical analysis of of 1 day-post-fertilization zebrafish embryo labeling Actin with ab209857 at 1/50 dilution.
ab209857 has been referenced in 1 publication.
- Wang L et al. ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p. Hum Cell 32:306-315 (2019). PubMed: 31104300