• Product name
  • Description
    Rabbit polyclonal to Actin
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Actin aa 365-375 (C terminal).


    Database link: P63261

  • Positive control
    • IHC-P: Rat cardiac muscle tissue. WB: Lysate from rat cardiac muscle tissue, rat brain tissue, rat testis tissue, rat skeletal muscle tissue. Whole cell lysate prepared from MM231 cells, HeLa cells, SMMC cells, HT1080 cells, SW620 cells.


Our Abpromise guarantee covers the use of ab95437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all


  • All lanes : Anti-Actin antibody (ab95437) at 0.5 µg

    Lane 1 : Mouse Brain Tissue Lysate at 50 µg
    Lane 2 : Mouse Spleen Tissue Lysate at 50 µg
    Lane 3 : Mouse Thymus Tissue Lysate at 50 µg
    Lane 4 : HEPA Whole Cell Lysate at 40 µg
    Lane 5 : NIH3T3 Whole Cell Lysate at 40 µg

    Predicted band size: 42 kDa

  • All lanes : Anti-Actin antibody (ab95437) at 0.5 µg/ml

    Lane 1 : Rat cardiac muscle missue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat testis tissue lysate
    Lane 4 : Rat skeletal muscle tissue lysate
    Lane 5 : MM231 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : SMMC cell lysate
    Lane 8 : HT1080 cell lysate
    Lane 9 : SW620 cell lysate

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • ab95437 at 2µg/ml staining Actin in rat cardiac muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed.


This product has been referenced in:
  • Wang S  et al. SIRT1 activation inhibits hyperglycemia-induced apoptosis by reducing oxidative stress and mitochondrial dysfunction in human endothelial cells. Mol Med Rep 16:3331-3338 (2017). Read more (PubMed: 28765962) »
  • Yu Q  et al. Silencing of FRAT1 by siRNA inhibits the proliferation of SGC7901 human gastric adenocarcinoma cells. Biomed Rep 4:223-226 (2016). Read more (PubMed: 26893843) »
See all 3 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Tissue lysate - whole (bones, skin, lung)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
50 µg
bones, skin, lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Submitted Mar 08 2016

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