Anti-Actin antibody (Biotin) (ab113279)


  • Product name
    Anti-Actin antibody (Biotin)
    See all Actin primary antibodies
  • Description
    Rabbit polyclonal to Actin (Biotin)
  • Host species
  • Conjugation
  • Specificity
    The epitope recognized by ab113279 maps to the N-terminus of Human Beta-actin using the numbering given in Swiss-Prot entry P60709 (GeneID 60). The N-terminus of Beta-Actin is highly conserved with Gamma-Actin and preliminary indications are that ab113279 also recognizes Gamma- Actin
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, a wide range of other species, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to Human Actin (N terminal).

  • Positive control
    • HeLa whole cell lysates.



Our Abpromise guarantee covers the use of ab113279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease
      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similarities
      Belongs to the actin family.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • a actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all


    • All lanes : Anti-Actin antibody (Biotin) (ab113279) at 0.07 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 42 kDa

      Exposure time: 30 seconds


    ab113279 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your patience.

    I just received an update form lab regarding the epitope for ab79667 and ab113279. Unfortunately the epitope has not been determined so the information is not available. I am sorry we will not be able to suggest th...

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    Thank you for contacting us.

    I can confirm that I am still waiting for information from lab. I will send you the epitoope details of ab79667 as soon as I receive them.

    The other possible combination of ab8227 in sandwich ELISA would b...

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    Merci pour votre appel de hier. Je suis désolée du retard de ma réponse.

    Voici le lien pour nos kits de conjugaision:

    et voici aussi le code pour le test promis:


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