• Product name
    Anti-Actin antibody [C4]
    See all Actin primary antibodies
  • Description
    Mouse monoclonal [C4] to Actin
  • Host species
  • Specificity
    ab14128 recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. ab14128 labels myotubes and stains myoblasts and fibroblasts, it does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, ab14128 shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. The clone number has been updated from (2Q1055) to (C4) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Chicken, Saccharomyces cerevisiae, Tetrahymena, Caenorhabditis elegans, Mammals, Dictyostelium discoideum, Physarum polycephalum, Vertebrata
  • Immunogen

    Recombinant fragment corresponding to Chicken Actin aa 50-70 (N terminal). The immuogen is purified chicken gizzard actin. This is predominantly smooth muscle gamma-actin. Is a pan-actin which recognizes all actin isoforms.


    Database link: P63270

  • Epitope
    The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.
  • Positive control
    • This antibody gave a positive signal in both Human Skeletal Muscle and Heart tissue lysates as well as the following whole cell lysates: A431; HEK293; MCF7; HeLa; MDA MB 231. ICC/IF: HeLa cells
  • General notes
    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.



Our Abpromise guarantee covers the use of ab14128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 20 µg/ml. Predicted molecular weight: 43 kDa.

On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kDa protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.

ICC 1/500.

Methanol fixed HeLa and NIH/3T3 cells


  • Function
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease
    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all


  • All lanes : Anti-Actin antibody [C4] (ab14128) at 1/1000 dilution

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-231 (Breast Carcinoma cell line)
    Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
    Lane 7 : Heart (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab14128 overnight at 4°C. Antibody binding was detected using ab175783 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • ab14128 staining Actin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab14128 at 5μg/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Mouse Alexa Fluor 488 secondary (ab150117) at 2 μg/ml (shown in green) and Goat anti-Rabbit Alexa Fluor 594 secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
    Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.


This product has been referenced in:
  • Gu L  et al. The exosome secretion inhibitor neticonazole suppresses intestinal dysbacteriosis-induced tumorigenesis of colorectal cancer. Invest New Drugs N/A:N/A (2019). Read more (PubMed: 30887254) »
  • Drazic A  et al. NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. Proc Natl Acad Sci U S A 115:4399-4404 (2018). Read more (PubMed: 29581253) »
See all 28 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Western blot
Chlamydomonas reinhardtii Cell lysate - whole cell (Chlamydomonas reinhardtii)
Loading amount
20 µg
Chlamydomonas reinhardtii
Various concentrations of calcium (0M to 10^-3M)
Gel Running Conditions
Non-reduced Denaturing (12% gradient gel (polyacrylamide))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 23°C

Caroline Lam

Verified customer

Submitted May 10 2013


Thank you for your reply.
We have beta actin antibodies that would fulfill all your criteria:
ab8227 (https://www.abcam.com/beta-Actin-antibody-Loading-Control-ab8227.html) - in case beta actin would be acceptable for your experiments.
Or ab75186 (https://www.abcam.com/beta-Actin-antibody-Loading-Control-ab75186.html).
Unfortunately, we do not carry an actin-antibody that was raised in rabbit and was tested in ICC.
But we have several rabbit anti-actin antibodies that were tested in WB and/or IHC-P: ab15263, ab95437 or ab1801.
If you like to try one of these in ICC/IF you would be eligible for our testing discount program.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This program applies to ELISA kits and other kits we offer.
Please let me know if I shall send you a testing discount code.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!

Read More


Thank you for your reply.
If the word "actin" is entered into the search box on our homepage (top right corner), you will obtain entries for 21 actin antibodies, 9 actin-loading control antibodies, etc.
We do not have a rabbit anti-actin antibody that has been tested in ICC/IF, but the following 2 mouse monoclonal antibodies have similar tested applications, tested species and immunogens as the discontinued ab14130.
ab3280: https://www.abcam.com/Actin-antibody-ACTN05-C4-ab3280.html
ab14128: https://www.abcam.com/Actin-antibody-2Q1055-ab14128.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!

Read More
Human Cell (Fibroblast)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Feb 11 2009

Western blot
Mouse Cell lysate - whole cell (MEF)
Loading amount
8000 cells
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 22 2009

Western blot
Schizosaccharomyces pombe Cell lysate - whole cell (cells were grown to 3.5x10 7 cells/ml)
Loading amount
175000 cells
cells were grown to 3.5x10 7 cells/ml
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 28 2007


Thank you for your enquiry. The list of species this antibody has been tested in is quite impressive. The description of the antibody claims it is "pan-actin" due the highly conserved nature of actin. The antibody has indeed been tested specifically in rat. The species tested were: Avian, Bovine, Burro, C. elegans, Canine, Chicken, Donkey, Drospohila, E. coli, Equine, Feline, Frog, Goat, Goose, Guinea Pig, Hamster, Human, Macaque, Mammal, Monkey(Rhesus), Mouse, Porcine, Rabbit, Rat, Rodent, Rooster, Sheep, Tetrahymena, Xenopus and Yeast. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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