Western blot abreview for Anti-Actin antibody [2Q1055]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Loading amount
8000 cells
Specification
MEF
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/3000

Detection

Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 30 kDa

Abcam user community

Verified customer

Submitted Jan 22 2009

Sign up