Western blot abreview for Anti-Actin antibody [2Q1055]

Good
Abreviews
Application
Western blot
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (cells were grown to 3.5x10 7 cells/ml)
Loading amount
175000 cells
Specification
cells were grown to 3.5x10 7 cells/ml
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBST

Secondary antibody

Name
Non-Abcam antibody was used: stabilised goat anti-mouse HRP-conjugated antibody
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 42-43 kDa

Additional data

Additional Notes
The detection system used was Pierce SuperSignal West Femto Maximum Sensitivity Substrate

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Submitted Aug 28 2007

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