Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
1. untreated, 2. 10 mM D,L-Hcy for 0.5 hrs, 3. 10 mM L-Met for 0.5 hrs, 4. 10 mM L-Leu for 0.5 hrs, 5. untreated, 6. 10 mM D,L-Hcy for 4 hrs, 7. 10 mM L-Met for 4 hrs, 8. 10 mM L-Leu for 4 hrs
Specification
S. cerevisiae
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: between 37 and 50 kDa
Abcam user community
Verified customer
Submitted Feb 26 2018