Recombinant Anti-Actin antibody [EPR16769] (Alexa Fluor® 647) (ab206278)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Actin antibody [EPR16769] (Alexa Fluor® 647)
See all Actin primary antibodies -
Description
Rabbit monoclonal [EPR16769] to Actin (Alexa Fluor® 647) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken
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Immunogen
Synthetic peptide within Human Actin aa 50-150. The exact sequence is proprietary.
Database link: P68133 -
Positive control
- ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16769 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Actin antibody [EPR16769] (ab179467)
- Anti-Actin antibody [EPR16769] (Alexa Fluor® 488) (ab206277)
- Anti-Actin antibody [EPR16769] (HRP) (ab207674)
- Anti-Actin antibody [EPR16769] (Alexa Fluor® 405) (ab207900)
- Anti-Actin antibody [EPR16769] (Alexa Fluor® 555) (ab208080)
- Anti-Actin antibody [EPR16769] (Alexa Fluor® 568) (ab208143)
- Anti-Actin antibody [EPR16769] - BSA and Azide free (ab219733)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab206278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 421534 Chicken
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
see all -
Alternative names
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (Alexa Fluor® 647) (ab206278)](https://www.abcam.com/ps/products/206/ab206278/Images/ab206278-265764-anti-actin-antibody-epr16769-alexa-fluor-647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16769] (Alexa Fluor® 647) (ab206278)Ab206278 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206278 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% Methanol (5 minutes).
Datasheets and documents
References
ab206278 has not yet been referenced specifically in any publications.
