Recombinant Anti-Actin antibody [EPR16875] (ab200658)
![Western blot - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250155-anti-actin-antibody-epr16875-western-blot.jpg "Western blot - Anti-Actin antibody [EPR16875] (ab200658)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16875] to Actin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
Related conjugates and formulations
Overview
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Product name
Anti-Actin antibody [EPR16875]
See all Actin primary antibodies -
Description
Rabbit monoclonal [EPR16875] to Actin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HepG2, HeLa, UMNSAH/DF-1, C6 and RAW 264.7 cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart and kidney lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16875 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
|
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| WB |
1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| ICC/IF |
1/1000.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100. |
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WB
1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
|
ICC/IF
1/1000. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 421534 Chicken
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
- SwissProt: P68133 Human
- SwissProt: P68134 Mouse
see all -
Alternative names
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
Images
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Western blot - Anti-Actin antibody [EPR16875] (ab200658)All lanes : Anti-Actin antibody [EPR16875] (ab200658) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : C6 (Rat glial tumor cells) cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250154-anti-actin-antibody-epr16875-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [EPR16875] (ab200658)Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Actin with ab200658 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200658 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Western blot - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250158-anti-actin-antibody-epr16875-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR16875] (ab200658)All lanes : Anti-Actin antibody [EPR16875] (ab200658) at 1/10000 dilution
Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250153-anti-actin-antibody-epr16875-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Actin antibody [EPR16875] (ab200658)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Actin with ab200658 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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![Western blot - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250157-anti-actin-antibody-epr16875-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR16875] (ab200658)Anti-Actin antibody [EPR16875] (ab200658) at 1/2000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-250156-anti-actin-antibody-epr16875-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR16875] (ab200658)Anti-Actin antibody [EPR16875] (ab200658) at 1/2000 dilution + UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-Actin antibody [EPR16875] (ab200658)](https://www.abcam.com/ps/products/200/ab200658/Images/ab200658-8-benefits-of-recombinant-antibodies.png)
Anti-Actin antibody [EPR16875] (ab200658)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab200658 has been referenced in 2 publications.
- Liu Q et al. Hsa_circ_0001073 targets miR-626/LIFR axis to inhibit lung cancer progression. Environ Toxicol 36:1052-1060 (2021). PubMed: 33475233
- Ji Y et al. Potential biomarkers Ang II/AT1R and S1P/S1PR1 predict the prognosis of hepatocellular carcinoma. Oncol Lett 20:208 (2020). PubMed: 32963614