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  1. Link

    actin-antibody-igx3831h-ab213251.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Recombinant

Recombinant Anti-Actin antibody [IGX3831H] (ab213251)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
  • Western blot - Anti-Actin antibody [IGX3831H] (ab213251)
  • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
  • Anti-Actin antibody [IGX3831H] (ab213251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX3831H] to Actin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

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Goat F(ab')2 Anti-Human IgG - (Fab')2 (DyLight® 594), pre-adsorbed (ab98602)

View more associated products

Overview

  • Product name

    Anti-Actin antibody [IGX3831H]
    See all Actin primary antibodies
  • Description

    Human monoclonal [IGX3831H] to Actin
  • Host species

    Human
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Actin aa 150-400. The exact sequence is proprietary.
    Database link: P68133

  • Positive control

    • ICC/IF: HeLa, NIH3T3 cells WB: Human and mouse colon, HeLa, Jurkat, NIH3T3, PANC1, C2C12 whole cell lysates and human skeletal muscle.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody.
    Human monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    IGX3831H
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) (ab200622)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 647) (ab200623)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
    • Rabbit Anti-Human IgG H&L (HRP) (ab6759)
  • Isotype control

    • Human IgG1, kappa - Isotype Control (ab206198)
  • Recombinant Protein

    • Recombinant Actin protein (Tagged) (ab235861)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

This product gave a positive signal in HeLa cells and NIH3T3 fixed with 4% formaldehyde

Notes
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

This product gave a positive signal in HeLa cells and NIH3T3 fixed with 4% formaldehyde

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58 Human
    • Entrez Gene: 11459 Mouse
    • Omim: 102610 Human
    • SwissProt: P68133 Human
    • SwissProt: P68134 Mouse
    • Unigene: 1288 Human
    • Unigene: 214950 Mouse
    • Alternative names

      • a actin antibody
      • a-actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
      Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

      Ab213251 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-Actin antibody [IGX3831H] (ab213251)
      Western blot - Anti-Actin antibody [IGX3831H] (ab213251)
      All lanes : Anti-Actin [IGX3831H] at 1 µg/ml

      Lane 1 : Human colon tissue lysate
      Lane 2 : Mouse colon tissue lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : Jurkat whole cell lysate
      Lane 5 : NIH3T3 whole cell lysate
      Lane 6 : PANC1 whole cell lysate
      Lane 7 : C2C12 whole cell lysate
      Lane 8 : Human skeletal muscle tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 42 kDa


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.  The membrane was then blocked for an hour using 3% milk before being incubated with ab213251 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)
      Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831H] (ab213251)

      Ab213251 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab213251 at a 5µg/ml concentration, then detected with a goat anti-human (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Anti-Actin antibody [IGX3831H] (ab213251)
      Anti-Actin antibody [IGX3831H] (ab213251)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab213251? Please let us know so that we can cite the reference in this datasheet.

    ab213251 has been referenced in 1 publication.

    • Fan X  et al. Mesenchymal stem cells regulate type 2 innate lymphoid cells via regulatory T cells through ICOS-ICOSL interaction. Stem Cells 39:975-987 (2021). PubMed: 33662168

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