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  1. Link

    actin-antibody-loading-control-ab1801.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-Actin antibody - Loading Control (ab1801)

  • Datasheet
  • SDS
Reviews (11)Q&A (30)References (186)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)

Key features and details

  • Rabbit polyclonal to Actin - Loading Control
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Actin antibody - Loading Control
    See all Actin primary antibodies
  • Description

    Rabbit polyclonal to Actin - Loading Control
  • Host species

    Rabbit
  • Specificity

    This antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Actin aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13771)

  • Positive control

    • HeLa whole cell lysate or mouse brain lysate. IHC-P - Human Colon FFPE tissue section
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Isotype/Loading Controls
    • Loading Controls
    • Actin
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Actin protein (Tagged) (ab235861)
  • Related Products

    • Wiskostatin, N-WASP inhibitor (ab141085)
    • CK 666, Actin polymerization inhibitor (ab141231)
    • Jasplakinolide, Actin polymerization and stabilization inducer (ab141409)
    • Cytochalasin E, actin-polymerization inhibitor (ab141788)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
      Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
      Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68133 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 421534 Chicken
      • Entrez Gene: 281592 Cow
      • Entrez Gene: 58 Human
      • Entrez Gene: 11459 Mouse
      • Entrez Gene: 29437 Rat
      • Entrez Gene: 407658 Zebrafish
      • Omim: 102610 Human
      • SwissProt: P68139 Chicken
      • SwissProt: P68138 Cow
      • SwissProt: P68133 Human
      • SwissProt: P68134 Mouse
      • SwissProt: P68135 Rabbit
      • SwissProt: P68136 Rat
      • Unigene: 1288 Human
      • Unigene: 214950 Mouse
      • Unigene: 82732 Rat
      see all
    • Alternative names

      • a actin antibody
      • a-actin antibody
      • ACTA antibody
      • ACTA1 antibody
      • Actin alpha skeletal muscle antibody
      • Actin antibody
      • actin, alpha 1, skeletal muscle 1 antibody
      • actin, alpha 1, skeletal muscle antibody
      • Actin, alpha skeletal muscle antibody
      • actina antibody
      • actine antibody
      • ACTS_HUMAN antibody
      • aktin antibody
      • Alpha Actin 1 antibody
      • Alpha skeletal muscle Actin antibody
      • alpha skeletal muscle antibody
      • alpha-actin antibody
      • Alpha-actin-1 antibody
      • ASMA antibody
      • CFTD antibody
      • CFTD1 antibody
      • CFTDM antibody
      • MPFD antibody
      • NEM1 antibody
      • NEM2 antibody
      • NEM3 antibody
      • nemaline myopathy type 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)

      IHC image of ab1801 staining Actin in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1801, 5μl/ml concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : NIH/3T3 whole cell lysate (ab7179)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)Image from PLoS One. 2014; 9(3): e92128. Fig 9,•DOI: 10.1371/journal.pone.0092128 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Western blot analysis of HeLa cells treated for 12 hours with hesperidin (h) (2.5 μg/ml, 4,01 μM), mangiferin (5 μg/ml, 11.84 μM) (m), and hesperidin (2.5 μg/ml, 4.01 μM) in a presence of mangiferin (5 μg/ml, 11.84 μM) (h+m). Immunoblotting was performed with the following primary antibodies: Bax (ab32503), BCL2 (ab59348), beta actin (ab1801), and caspase 8. After the washing steps, the membranes were incubated with goat anti-rabbit IgG (H+L) or with goat anti-mouse IgG (H+L) HRP-conjugated secondary antibodies and detected using ECL. Densitometry was performed using Image Lab software v. 4.1 (BioRad).

      Top panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the mRNA levels were monitored in real - time PCR experiments. The BAX and BCL2 mRNA levels results from 2 independent experiments (n?=?8) are plotted relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 18S rRNA levels and expressed as a fold change over the EtOH control. Error bars represent standard derivations.

      Bottom panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the protein levels of Bax and BCL2 were detected with SDS-PAGE and Western Blot and related to beta actin levels.

    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
      Lane 2 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa


      Exposure time: 30 seconds
    • Western blot - Anti-Actin antibody - Loading Control (ab1801)
      Western blot - Anti-Actin antibody - Loading Control (ab1801)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution

      All lanes : Whole cell lysates prepared from HUVEC cells

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 42 kDa


      Exposure time: 30 seconds

      See Abreview

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (186)

    Publishing research using ab1801? Please let us know so that we can cite the reference in this datasheet.

    ab1801 has been referenced in 186 publications.

    • Qiao W  et al. miR-224-5p protects dental pulp stem cells from apoptosis by targeting Rac1. Exp Ther Med 19:9-18 (2020). PubMed: 31897093
    • Li Y & Chen Z The oncogenic role of microRNA-500a in colorectal cancer. Oncol Lett 19:1799-1805 (2020). PubMed: 32194673
    • Imamichi H  et al. Defective HIV-1 proviruses produce viral proteins. Proc Natl Acad Sci U S A 117:3704-3710 (2020). PubMed: 32029589
    • Korbut AI  et al. SGLT2 Inhibitor Empagliflozin and DPP4 Inhibitor Linagliptin Reactivate Glomerular Autophagy in db/db Mice, a Model of Type 2 Diabetes. Int J Mol Sci 21:N/A (2020). PubMed: 32340263
    • Yoshida S  et al. Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes. Nat Commun 11:2652 (2020). PubMed: 32461611
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 11 Abreviews

    Western blot abreview for Anti-Actin antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cancer cell lines)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    15 µg
    Specification
    cancer cell lines
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 03 2019

    Western blot abreview for Anti-Actin antibody - Loading Control

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (RIPA lysate of whole lung tissue)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    100 µg
    Specification
    RIPA lysate of whole lung tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Ben Thomson

    Verified customer

    Submitted Aug 14 2015

    Western blot abreview for Anti-Actin antibody - Loading Control

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient gel)
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Specification
    HeLa cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 21 2014

    Immunocytochemistry/ Immunofluorescence

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    African Green Monkey Cell (COS7)
    Specification
    COS7
    Permeabilization
    Yes - Triton X 100 0.3%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 03 2013

    Western blot abreview for Anti-Actin antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ovarian cancer cells)
    Loading amount
    30 µg
    Specification
    ovarian cancer cells
    Gel Running Conditions
    Reduced Denaturing (10% gel, discontinuous)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 07 2012

    Western blot abreview for Anti-Actin antibody - Loading Control

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Brain)
    Loading amount
    2.25 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (10% resolving SDS-PAGE gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Fusun Doldur Balli

    Verified customer

    Submitted May 03 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Actin antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mammary epithelial)
    Specification
    Mammary epithelial
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS, 5 min
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Virginia Spencer

    Verified customer

    Submitted Mar 10 2011

    Western blot abreview for Anti-Actin antibody - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Loading amount
    10 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2009

    Western blot abreview for Anti-Actin antibody - Loading Control

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Umbilical Vein Endothelial Cell)
    Loading amount
    15 µg
    Specification
    Human Umbilical Vein Endothelial Cell
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 17 2008

    Western blot abreview for Anti-Actin antibody - Loading Control

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain tissue)
    Loading amount
    30 µg
    Specification
    brain tissue
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 26 2008

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