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We ordered anti-actin rabbit polyclonal antibody (ab1801, ordered 8/31/12) and have not been able to get the antibody
to detect actin in a Western blot. We have followed the instructions for the antibody, which includes blocking in 5%
BSA and using a 1/1000 dilution. We have tried not only BSA, but 5% milk (even though the instructions state that milk reduces the signal). We have also tried a 1/500 dilution for the antibody. We have checked that the protein has transferred well using PonceauS stain. We have tried the Western using different anti-actin antibody as a control and have been able to detect actin. We have tried the Western with ab1801 several times with no luck.
Do you have other suggestions for the antibody? Would it be possible to get a refund or replacement for this antibody?
It is quite frustrating to do a Western blot where the experiment seems to be working, but our results are inconclusive because our actin control is not working.
Asked on Oct 25 2012
Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Could I just ask to confirm the species that your samples were from (i.e. mouse)?
As long as the antibody was being used in a tested species, I would be happy to offer you a replacment or refund. Please let me know which you would prefer.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.
Answered on Oct 25 2012